Extracellular potassium can simulate the role of serum as an activator of uridine uptake by quiescent hamster cells in culture

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Abstract

Replacement of ~100 mM of sodium chloride in the extracellular medium of quiescent hamster fibroblasts (Nil 8 and BHK cells) by potassium chloride causes an increase in the rate of uridine uptake. This increase is identical with that achieved by addition of 10% serum to the same cultures. The effects of serum and KCl are not additive. The dependence of the rate of uridine uptake on extracellular KCl concentration is of a sigmoid nature. The time course of the activation process is similar to that of serum activation of uridine uptake in the same cells. The high rate of uridine uptake persists for at least 30 min after return to an extracellular medium containing a high concentration of sodium.

References (16)

  • JD Hare

    Biochim biophys acta

    (1972)
  • E Rozengurt et al.

    Biochim biophys acta

    (1977)
  • R Koren et al.

    FEBS lett

    (1978)
  • O Heichal et al.

    Biochim biophys acta

    (1979)
  • MD Hollenberg et al.

    J biol chem

    (1975)
  • R Koren et al.

    FEBS lett

    (1979)
  • WR Lieb et al.

    Biochim biophys acta

    (1974)
  • DD Cunningham et al.
There are more references available in the full text version of this article.

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This work was supported by Grant BCT 8 from the Bundesministerium für Forschung und Technology, West Germany, and by a grant from the Joint Research Fund of the Hebrew University and Hadassah Medical Center, Israel.

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