Elsevier

Developmental Biology

Volume 45, Issue 1, July 1975, Pages 112-136
Developmental Biology

Full paper
Regulation after proximal or distal transposition of limb regeneration blastemas and determination of the proximal boundary of the regenerate

https://doi.org/10.1016/0012-1606(75)90246-8Get rights and content

Abstract

When blastemas of several stages of differentiation were grafted in normal orientation to stump levels proximal or distal to their level of origin, normal limbs regenerated. Histological and autoradiographic studies of the development of these regulated limbs showed that the grafted blastemas formed only structures normally distal to their level of origin. In the case of a blastema transplanted proximally, regulation occurred by intercalary regeneration from the stump, whereas, when blastemas were transplanted distally, regulation appeared to take place within the blastema itself by a distal shift in its pattern of organization. The results suggest that the proximal limit of the limb regenerate is determined by level-specific properties of the limb cells but that these properties allow for interactions leading to regulation when different levels of stump and blastema are brought together.

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    This research was supported by the National Science Foundation, Grant No. BMS 71-01579 A01.

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