Calculation factors and reference values for quantitative lipoprotein electrophoresis in childhood

doi:10.1016/0009-8981(82)90409-0

Clinica Chimica Acta

Volume 119, Issues 1–2, 26 February 1982, Pages 99-105

https://doi.org/10.1016/0009-8981(82)90409-0 Get rights and content

Abstract

Agarose gel electrophoresis of lipoproteins combined with quantitative densitometry (Lipidophor ®) and total cholesterol determination enables the calculation of α-, pre-β- and β-cholesterol. This method is quick, precise and requires less than 100 μl serum. The calculation factors originally derived from adults were re-evaluated and corrected for children. This was achieved by iterative calculation of the results obtained by quantitative densitometry and by enzymatic determination of the cholesterol contents in electrophoretically separated lipoprotein bands. Thus, calculation factors for children were obtained, which were used for the evaluation of reference values for α-, pre-β- and β-cholesterol and for the β/α-lipoprotein ratio based on a reference group of 575 healthy children of 5–18 years of age.

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There are more references available in the full text version of this article.

Cited by (1)

Cholesterol fractions and triglycerides in children and adults with down's syndrome
1984, Clinica Chimica Acta
In 186 patients with Down's syndrome (age 1–68 yr) total cholesterol and triglycerides were determined, quantitative lipoprotein electrophoresis was performed and cholesterol fractions and β/a-lipoprotein ratios were calculated. A reference group of 51 non-mongoloid mentally handicapped adults living in the same institutions were examined in the same way. Patients' total cholesterol, β-cholesterol and triglycerides did not differ from the controls. However, αcholesterol was significantly lower and the β/α-lipoprotein ratio significantly higher in patients, findings which are associated in the general population with a high risk for premature atherosclerosis. Interestingly, mortality causes and pathological findings in Down's syndrome show no increased frequency of cardiovascular diseases.

^☆: Partly presented at the First Joint Meeting of the British, German and Dutch Societies for Clinical Chemistry in Noordwijkerhout (The Netherlands), 9–10.4.1981.

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Calculation factors and reference values for quantitative lipoprotein electrophoresis in childhood☆

Abstract

Clin ChimActa

Improved method for the enzymatic microdetermination of cholesterol in plasma lipoproteins

J Clin Chem Clin Biochem

Fortschritte in der Analytik des Lipoproteinmusters

Inn Med

Serum lipids and lipoproteins at birth in a biracial population: the Bogalusa Heart Study

Pediatr Res

Nature of the interaction between low-density lipoproteins and polyanions and metal ions as exemplified by heparin and Ca2+

Clin Chem

Nature of the interaction between low-density lipoproteins and polyanions and metal ions as exemplified by heparin and Ca²⁺