Elsevier

Cellular Immunology

Volume 116, Issue 1, 1 October 1988, Pages 73-85
Cellular Immunology

Differential immunomodulation by anti-CD2 monoclonal antibodies of anti-CD3-induced T cell activation: Dependence upon the individual anti-CD3 monoclonal antibody used for activation

https://doi.org/10.1016/0008-8749(88)90211-0Get rights and content

Abstract

Two monoclonal antibodies (mAb) recognizing different CD2 epitopes each inhibited anti-CD3-induced proliferation and anti-CD3-induced increase in surface CD2 expression. The magnitude of inhibition by either anti-CD2 mAb was dependent upon which anti-CD3 mAb was used as the stimulus, being more pronounced when the anti-CD3 mAb 454 was used as the stimulus than when either anti-CD3 mAb 147 or 446 was the stimulus. The effects of neuraminidase-treated sheep erythrocytes (which bind to CD2) were also more pronounced on mAb 454-induced proliferation than on mAb 147- or 446-induced proliferation. Furthermore, the effects of preincubation with anti-CD2 mAb depended upon the responder status of the donor to IgG1 anti-CD3 mAb. Preincubation of high-responder cells with anti-CD2 mAb had little effect on subsequent IgG1 anti-CDS-induced proliferation. In contrast, preincubation of low-responder cells with anti-CD2 mAb usually augmented the otherwise small proliferative response to IgG1 anti-CD3 mAb. Taken together, these observations suggest that interaction of surface CD2 with ligand alters the response of T cells to anti-CD3 mAb, but these effects depend upon the individual anti-CD3 mAb used for stimulation. These studies raise the possibility that perturbation of different parts of the CD3-T cell antigen receptor complex may lead to different sequelae, and, as a result, the T cell may respond to a given immunomodulator in different ways.

References (55)

  • S.C. Meuer et al.

    Cell

    (1984)
  • J.P. Van Wauwe et al.

    Cell. Immunol

    (1984)
  • G.M. Kammer et al.

    Cell. Immunol

    (1984)
  • J.P. Van Wauwe et al.

    Cell. Immunol

    (1983)
  • J.T. Kurnick et al.

    Clin. Immunol. Immunopathol

    (1983)
  • E.C. Ebert

    Clin. Immunol. Immunopathol

    (1985)
  • M.B. Brenner et al.

    Cell

    (1985)
  • H.C. O'Neill et al.

    Cell

    (1987)
  • S.C. Meuer et al.

    Nature (London)

    (1983)
  • J.P. Van Wauwe et al.

    J. Immunol

    (1980)
  • T.W. Chang et al.
  • P. Brottier et al.

    J. Immunol

    (1985)
  • W. Holter et al.

    J. Exp. Med

    (1986)
  • D. Olive et al.

    Eur. J. Immunol

    (1986)
  • J. Van Wauwe et al.

    Immunology

    (1981)
  • R. Palacios et al.

    J. Immunol

    (1982)
  • W. Verbi et al.

    Eur. J. Immunol

    (1982)
  • A. Moretta et al.

    Eur. J. Immunol

    (1986)
  • W. Stohl et al.

    J. Immunol

    (1987)
  • H. Kaneoka et al.

    J. Immunol

    (1983)
  • W.J.M. Tax et al.

    Nature (London)

    (1983)
  • C.Y. Wang et al.

    Hybridoma

    (1986)
  • D.N. Posnett et al.

    J. Exp. Med

    (1984)
  • D.N. Posnett et al.

    Scand. J. Immunol

    (1984)
  • W. Stohl et al.

    Scand. J. Immunol

    (1984)
  • M.L. Plunkett et al.

    J. Immunol

    (1986)
  • M. Kamoun et al.

    J. Exp. Med

    (1981)
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