Proline hydroxylation by cell free extract of a streptomycete

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Abstract

Free L-proline was hydroxylated to free L-hydroxyproline by cell free extract of Streptomycesgriseoviridus P8648. The hydroxylation reaction required ferrous ion, 2-ketoglutarate and ascorbate. Zinc ion, ethylenediaminetetraacetic acid and α,α′-dipyridyl inhibited the reaction. Optimum temperature and pH were 25.0°C and 7.5, respectively.

References (8)

  • E. Katz et al.

    J. Biol. Chem

    (1962)
  • E. Katz et al.

    J. Biol. Chem

    (1979)
  • A. Bensadoun et al.

    Anal. Biochem

    (1976)
  • J.C. Sheehan et al.

    J. Am. Chem. Soc

    (1959)
There are more references available in the full text version of this article.

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