The host factor requirement of Qβ RNA replicase

doi:10.1016/0005-2787(74)90289-5

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis

Volume 366, Issue 3, 28 October 1974, Pages 292-299

https://doi.org/10.1016/0005-2787(74)90289-5 Get rights and content

Abstract

It has been reported that Qβ RNA-directed RNA synthesis by Qβ replicase requires two host-specific factors, HF I and HF II. While HF I is a specific protein, a variety of basic proteins show HF II activity. We offer evidence that the requirement for HF II is due to an inhibitor present in certain Qβ RNA preparations and that this inhibitor is counteracted by basic proteins.

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^∗: Present address: Imperial Cancer Research Fund Laboratories, Dept. of Molecular Virology, London WC2A 3PX.

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The host factor requirement of Qβ RNA replicase

Abstract

Biochim. Biophys. Acta

J. Mol. Biol.

Nature

Nature

Nature