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Identification of the molecular species of lysophosphatidic acid produced when platelets are stimulated by thrombin

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Abstract

Platelets, when stirred with 3 U thrombin/109 platelets, produced significant quantities of palmitoyllysophosphatidic acid (2.17 ng/109 platelets), stearoyllysophosphatidic acid (2.11 ng/109 platelets), and arachidonoyllysophosphatidic acid (1.06 ng/109 platelets). When platelets were pretreated with 100 μM of the phospholipase A2 inhibitor U10029A, there was a significant decrease in thrombin-stimulated production of stearoyllysophosphatidic acid (to 0.16 ng/109 platelets), while arachidonoyllysophosphatidic acid production was unchanged. U10029A concomitantly increased thrombin-stimulated production of stearoyl-containing phosphatidic acid species (primarily stearoylarachidonoylphosphatidic acid) from 5.99 to 9.71 ng/109 platelets. The results are consistent with the concept that stearoyllysophosphatidic acid production in platelets occurs via phospholipase A2 degradation of phosphatidic acid.

References (17)

  • G. Mauco et al.

    Biochimie (Paris)

    (1978)
  • J.M. Gerrard et al.

    Biochim. Biophys. Acta

    (1984)
  • A.M. Benton et al.

    Blood

    (1982)
  • M.M. Billah et al.

    J. Biol. Chem.

    (1981)
  • D.P. Wallach et al.

    Biochem. Pharmacol.

    (1981)
  • M.M. Billah et al.

    J. Biol. Chem.

    (1982)
  • M.J. Broekman et al.

    J. Biol. Chem.

    (1981)
  • G. Mauco et al.

    FEBS Lett.

    (1979)
There are more references available in the full text version of this article.

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