Adriamycin-induced changes in the surface membrane of sarcoma 180 ascites cells

https://doi.org/10.1016/0005-2736(81)90421-1Get rights and content

Abstract

Adriamycin increases (a) the rate of agglutination of Sarcoma 180 cells by concanavalin A after brief exposure of 2–3 h and (b) membrane fluidity as measured by ESR within 30 min of exposure at concentrations of the anthracycline of 10−7–10−5 M. The effect of adriamycin on agglutination is not due to an increase in the number of surface receptors for concanavalin A, since the extent of binding of the lectin is not altered by adriamycin and no change occurs in the rate of occupancy of the concanavalin A binding sites by the lectin in cells treated with the antibiotic. The order parameter, a measurement of membrane fluidity, decreases in cells exposed to adriamycin and is dose-related. The results indicate that adriamycin can induce changes in the surface membrane of Sarcoma 180 cells within a brief period of exposure to a low but cytotoxic level of this agent.

References (39)

  • F. Zunino et al.

    Biochim. Biophys. Acta

    (1977)
  • T. Facchinetti et al.

    Chem. Biol. Interact.

    (1978)
  • P.M. Kanter et al.

    Leukemia Res.

    (1979)
  • A. Necco et al.

    Pharmacol. Res. Commun.

    (1976)
  • M. Menozzi et al.

    Biochem. Biophys. Res. Commun.

    (1978)
  • R. Goldman et al.

    Biochim. Biophys. Acta

    (1978)
  • M. Duarte-Karim et al.

    Biochem. Biophys. Res. Commun.

    (1976)
  • E. Goormaghtigh et al.

    Biochim. Biophys. Acta

    (1980)
  • E. Goormaghtigh et al.

    Biochem. Pharmacol.

    (1980)
  • S.A. Murphree et al.

    Biochem. Pharmacol.

    (1976)
  • T.R. Tritton et al.

    Biochem. Pharmacol.

    (1977)
  • T.R. Tritton et al.

    Biochem. Biophys. Res. Commun.

    (1978)
  • B.L. Bales et al.

    Biochim. Biophys. Acta

    (1977)
  • B. Storrie et al.

    Cell

    (1977)
  • J.L. Wang et al.

    J. Biol. Chem.

    (1978)
  • R.D. Klausner et al.

    J. Biol. Chem.

    (1980)
  • B.L. Bales et al.

    Biochim. Biophys. Acta

    (1978)
  • A. DiMarco
  • S. Mong et al.

    Mol. Pharmacol.

    (1980)
  • Cited by (0)

    View full text