A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: Application to quantitation of β-galactosidase in lacZ-transfected cells
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Microbially derived biosensors for diagnosis, monitoring, and epidemiology for future biomedicine systems
2020, New and Future Developments in Microbial Biotechnology and Bioengineering: Trends of Microbial Biotechnology for Sustainable Agriculture and Biomedicine Systems: Perspectives for Human HealthMetal-to-ligand charge-transfer: Applications to visual detection of β-galactosidase activity and sandwich immunoassay
2017, TalantaCitation Excerpt :Consequently, it is significantly important to develop simple and practical methods for the detection of β-Gal activity. Several approaches, such as colorimetric [7–10], fluorometric [11–13], surface-enhanced Raman scattering (SERS) [14], magnetic resonance imaging (MRI) [15,16], and luminescent methods [17,18], have been established in laboratories for the detection of β-Gal activity. In recent years, the colorimetric approach has attracted considerable attention in the fields of chemical and biological sensing by virtue of its simplicity and practicality.
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2017, Sensors and Actuators, B: ChemicalFructose 1-phosphate is the one and only physiological effector of the Cra (FruR) regulator of Pseudomonas putida
2014, FEBS Open BioCitation Excerpt :On the contrary, when CraPP metabolic agonists release repression, the lacZ fusion is transcribed and the reporter is expressed. In order to measure accurately β-galactosidase we adopted a variant of the Miller assay [20] that uses the super-sensitive β-Galacto-Light PlusTM luminiscent substrate of the enzyme [21]. Since P. putida cannot internalize phosphorylated sugars F1P/FBP, these effectors could not be added directly to the medium for examining their action in vivo.
Genetically modified whole-cell bioreporters for environmental assessment
2013, Ecological IndicatorsCitation Excerpt :Commercially available kits such as the SOS Chromotest implement lacZ-based fusions to DNA responsive genes to monitor environmental samples for potential mutagenic or carcinogenic genotoxic compounds (Quillardet et al., 1982). Besides colorimetric endpoints, lacZ bioreporters can be used in conjunction with a variety of β-galactoside substrates that permit luminescent (Nazarenko et al., 2001), chemiluminescent (Jain and Magrath, 1991), or fluorescent (Rowland et al., 1999) endpoints. A disadvantage of lacZ-based biosensing strategies is that β-gal is endogenously present in natural environmental matrices and contributes to elevated background activity.
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