Elsevier

Analytical Biochemistry

Volume 199, Issue 1, 15 November 1991, Pages 119-124
Analytical Biochemistry

A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: Application to quantitation of β-galactosidase in lacZ-transfected cells

https://doi.org/10.1016/0003-2697(91)90278-2Get rights and content

Abstract

An optimized chemiluminescent assay for β-galactosidase using a chemiluminescent substrate AMPGD (3-(4-methoxyspiro[1,2-dioxetane-3,2′-tricyclo-[3.3.1.13,7]decan]-4-yl)phenyl-β-d-galactopyranoside) is described. This assay is rapid and sensitive and can detect as little as 2 fg of β-galactosidase. Its use for the quantitation of β-galactosidase in cells transfected with lacZ-expressing vectors is described. It is possible to detect a single cell stably expressing lacZ by this technique.

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    Experiments in Molecular Genetics

There are more references available in the full text version of this article.

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