Improved estimation of DNA fragment lengths from agarose gels☆
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2005, ICES Journal of Marine ScienceCitation Excerpt :Restriction fragments were size-separated on 6% acrylamide (acrylamide:bisacrylamide 30% w/v 37, 5:1, NBL Gene Science Limited, Northumberland, UK) gels in 1× TBE buffer (Sambrook et al., 1989) at 200 V, 30 mA/gel, during 2–2:30 h, and silver stained (Sambrook et al., 1989). Fragment sizes were estimated with the program DNAFRAG (Schaffer and Sederoff, 1981) using pGEM® as a molecular marker. MtDNA haplotype patterns for each enzyme were given an alphabetical (A, B, etc.) code, with haplotype A being the most common, and the remainder ranked in the order in which they were found for each restriction enzyme.
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The work was supported in part by U. S. Public Health Service Grants GM 11546 and GM 26267. This is Paper 6622 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, N. C.