Permeabilization of yeast for in situ determination of α-glucosidase

doi:10.1016/0003-2697(81)90064-6

Analytical Biochemistry

Volume 113, Issue 1, 1 May 1981, Pages 188-192

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Abstract

A quantitative in situ assay of yeast α-glucosidase involving permeabilization of the cells by freezing and thawing is described. The assay was applied to different strains in different physiological states and was shown to give results comparable to those obtained with total cell homogenates. The primary advantage of the in situ assay was the possibility of analyzing a large number of samples from the same culture during a growth curve using a very reduced cell mass.

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Permeabilization of yeast for in situ determination of α-glucosidase

Abstract

Biochem. Biophys. Res. Commun

Anal. Biochem

Anal. Biochem

Anal. Biochem

Anal. Biochem

J. Biol. Chem

Eur. J. Biochem

Biochem. Biophys. Res. Commun

Biochim. Biophys. Acta