A simple micro-assay for inorganic phosphate
References (15)
Anal. Biochem
(1970)- et al.
Clin. Chim. Acta
(1966) - et al.
J. Biol. Chem
(1964) - et al.
- et al.
Ukr. Kim. Zh
(1967) - et al.
Anal. Chem
(1971) - et al.
Anal. Chem
(1949)
Cited by (122)
The pattern of apolipoprotein A-I lysine carbamylation reflects its lipidation state and the chemical environment within human atherosclerotic aorta
2022, Journal of Biological ChemistryCitation Excerpt :The amount of cholesterol was determined using a LiquiColor enzymatic cholesterol test kit (Stanbio Laboratory). Total phospholipid content in the rHDL was quantified by microphosphorous assay (80). Protein (either in lipid-poor form or within an HDL particle) was subjected to in vitro carbamylation by one of two methods: (1) carbamylation with the complete MPO/H2O2/SCN- system (30 nM MPO, 100 μM SCN−) using a molar ratio of 10 mol of H2O2 to 1 mol of apoA-I (apoA-I molecular weight used for calculations = 28,300 kDa) and (2) carbamylation by CNO− carried out using 10 mol of KCNO for each mol of apoA-I, in 50 mM sodium phosphate (pH 7.0), at 37 °C.
An improved malachite green assay of phosphate: Mechanism and application
2011, Analytical BiochemistryCitation Excerpt :In the acidified reaction solutions of phosphate, all the tested hydrophobic amines of interference and MLG become hydrophobic cations, but all the other tested hydrophobic amines of negligible interference have weak hydrophobicity and/or negligible charges (Fig. S1, Supplementary materials). Principally via ionic, hydrophobic interactions in the acidified reaction solutions, phosphomolybdate and hydrophobic amine dyes including MLG and rhodamine form the complexes that have a strong tendency to aggregate and precipitate but can be stabilized by surfactants like PVA [1–13]. Therefore, the following mechanism may account for the interference of the tested hydrophobic amines. (
Surfactant-sensitized malachite green method for trace determination of orthophosphate in aqueous solution
2006, Analytica Chimica ActaCitation Excerpt :Table 2 summarizes the optimization of the method. It should be noted that, by taking advantage of micelle formation, the chemicals used in this method is substantially reduced those of the previous methods [10–16]. Based on the results of the optimization of reagent recipe, the final sample and reagent mixture contains 0.1 M HCl, 1.0 μM malachite green (MG), 2.3 mM molybdate, and 32 ppm Ultrawet 60 L.
Mechanism of cytosol phospholipase C and sphingomyelinase-induced lysosome destabilization
2006, BiochimieCitation Excerpt :The lower phase was evaporated and digested with 0.5 ml 70% perchloric acid at 190 °C for 120 min. Measurements of phosphorus were carried out as described in [22]. Briefly, 10 μl of digested sample was mixed with assay solution (0.9 ml 2.5 M HCl, 0.9 ml 26 mg/ml sodium molybdate and 0.5 ml water).