Collagen fractionation: Separation of native types I, II and III by differential prectipitation

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Abstract

Collagen types I, II and III can be purified in their native state from heterogeneous collagen solutions by fractional precipitation at neutral pH using ammonium sulfate, sodium chloride and ethanol as precipitants. This method of collagen separation is useful as a preparative procedure and should also serve as an analytical tool for identification of unknown radioactively labeled collagens.

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This is publication No. 678 of the Robert W. Lovett Memorial Group for the Study of Diseases Causing Deformities. This investigation was supported by a grant from the NIH (AM 3564) and from the American Cancer Society, Massachusetts Division (No. 1441-C-1).

2

Recipient Faculty Research Award, American Cancer Society (PR 107).

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