New correction factors for spectrophotometric assay of erythrocyte porphyrins

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Abstract

A reliable and convenient spectrophotometric method for the assay of uroporphyrin, coproporphyrin, and protoporphyrin is described. Equations were derived which correct for impuritie(s) isolated with the porphyrins from red cell hemolysates. The need for extensive purification of the porphyrin solutions was eliminated.

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