Biochemical and Biophysical Research Communications
Ageing increases SOCS-3 expression in rat hypothalamus: effects of food restriction
Section snippets
Materials and methods
Animals. Wistar albino male rats aged 3, 7, and 23 months were used throughout the study. The animals were obtained from the “Centro de Biologı́a Molecular Severo Ochoa” (Madrid, Spain). Rats were housed individually in climate-controlled quarters with 12 h light cycle and handled following the European Union laws and NIH guidelines. Animals were fed standard laboratory chow. Special care was taken to minimize animal suffering and to reduce the number of animals used.
Food restriction. Four- and
Results and discussion
In agreement with previous results [6], Table 1 shows that body weight increases in Wistar rats with ageing without significant changes in obesity Lee index. Aged rats remain normoglycemic but exhibit a progressive and marked increase in plasma leptin concentrations. Food restriction during 3 months keeps body weight to about 75–80% of ad libitum fed aged-mates and elicits a significant decrease of obesity Lee index in 7- and 23-month-old rats. Additionally, food restriction brings plasma
Acknowledgements
S.P. and N.G. were supported by Grant 1FD1997-1754-C03-02. Financial aid from Quı́mica Farmacéutica Bayer (Spain) is acknowledged. The Centro de Biologı́a Molecular is the recipient of institutional aid from the Ramon Areces Foundation. This work was supported by Grants 1/2FD1997-1754-C03 and PIGE-02-17 from MCYT and URJC, respectively (Spain).
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