Neuron
Volume 65, Issue 3, 11 February 2010, Pages 373-384
Journal home page for Neuron

Article
Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132

https://doi.org/10.1016/j.neuron.2010.01.005Get rights and content
Under an Elsevier user license
open archive

Summary

MicroRNAs (miRNAs) are noncoding RNAs that suppress translation of specific mRNAs. The miRNA machinery interacts with fragile X mental retardation protein (FMRP), which functions as translational repressor. We show that miR-125b and miR-132, as well as several other miRNAs, are associated with FMRP in mouse brain. miR-125b and miR-132 had largely opposing effects on dendritic spine morphology and synaptic physiology in hippocampal neurons. FMRP knockdown ameliorates the effect of miRNA overexpression on spine morphology. We identified NMDA receptor subunit NR2A as a target of miR-125b and show that NR2A mRNA is specifically associated with FMRP in brain. In hippocampal neurons, NR2A expression is negatively regulated through its 3′ UTR by FMRP, miR-125b, and Argonaute 1. Regulation of NR2A 3′UTR by FMRP depends in part on miR-125b. Because NMDA receptor subunit composition profoundly affects synaptic plasticity, these observations have implications for the pathophysiology of fragile X syndrome, in which plasticity is altered.

Highlights

► A large set of microRNAs are biochemically associated with FMRP in mouse brain ► miR-125b promotes thinner spines and weaker synapses; miR-132 induces opposite changes ► The NMDA receptor subunit NR2A is regulated by FMRP and miR-125b through its 3′ UTR ► These effects involve a functional interaction between these miRNAs and FMRP

CELLBIO
MOLNEURO
RNA

Cited by (0)

4

Present address: German Centre for Neurodegenerative Diseases (DZNE), 80336 Munich, Germany