Food allergy, dermatologic diseases, and anaphylaxisCharacterization of FcεRI-bearing CD123+ blood dendritic cell antigen-2+ plasmacytoid dendritic cells in atopic dermatitis☆
Section snippets
Methods
For the section “Reagents,” please see the ancillary material in the Journal's Online Repository at www.mosby.com/jaci.
CD123+BDCA-2+ pDCs in the peripheral blood of patients with AD display different phenotypical characteristics
First, we evaluated phenotypical characteristics of CD123+BDCA-2+ pDCs in the peripheral blood of patients with AD and healthy, nonatopic volunteers in detail by flow cytometry. BDCA-2+CD123+ pDCs freshly isolated from the blood of patients with AD expressed higher amounts of the costimatory molecule CD80 and the MHC I molecule on their cell surface than BDCA-2+CD123+ pDCs of healthy, nonatopic volunteers (Fig 1). In contrast, surface expression of L-selectin CD62L and cutaneous lymphocyte
Discussion
Although it is generally agreed that pDCs are involved in the elicitation of TH2-type immune responses, the exact mechanisms underlying this process have not been elucidated so far.1 Here we show for the first time that CD123+BDCA-2+ pDCs in the peripheral blood of patients with AD express enhanced amounts of the trimeric variant of FcεRI, which, similar to myeloid DCs, consists of the IgE-binding α-chain and the γ-chain dimer and is mostly occupied with IgE molecules.18 In contrast with the
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2015, Journal of Steroid Biochemistry and Molecular BiologyCitation Excerpt :These IC (CD11c+/CD14+/CD83+/CD123−/low+) were characterized by simultaneous expression of a MC marker, CD14, and DC marker, CD11c [9]. Other maturity and activity markers of DC, CD83 [1] and CD123 [28], were decreased in IC. On the contrary, no supplementation of 25(OH)D3 led to an expected, normal differentiation of MC to DC with downregulation of the monocytic marker CD14 and upregulation of CD11c [3].
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Supported by grants from the Deutsche Forschungsgemeinschaft (DFG NO/454-1-1 and DFG NO/454-2-1) and Bonner Forschungsförderung and by the grant Y078GEN of the Austrian Science Fund and the Forschungsgzentrum für Molekulare Medizin project of the Austrian Academy of Science.