Biochemical and Biophysical Research Communications
In vitro model to estimate gut inflammation using co-cultured Caco-2 and RAW264.7 cells
Section snippets
Materials and methods
Reagents. Dulbecco’s Modified Eagle’s Medium (DMEM, glutamine, low glucose), lipopolysaccharide (LPS) from E. coli O127, and recombinant murine TNF-α were purchased from Wako Pure Chemical Industries (Osaka, Japan). MEM (Eagle’s Minimum Essential Medium) was purchased from Nissui Pharmaceutical Co. Ltd., (Tokyo, Japan). RPMI 1640 medium and MEM non-essential amino acids (NEAA) were purchased from Gibco BRL (Grand Island, NY, USA). DMEM (glutamine, high glucose) and budesonide were obtained from
Establishment of a gut inflammation model using Caco-2 cells and RAW264.7 cells stimulated with LPS
An abnormal immunological response of intestinal immune cells to enteric microflora and any food substances is a critical factor driving IBD in genetically susceptible hosts [24], [25]. It has been reported that the inflamed tissue in IBD patients contains a large number of immune cells producing pro-inflammatory cytokines in excess [26]. IL-8 was secreted excessively by a variety of cells at the site of inflammation such as intestinal epithelial cells in IBD [4]. LPMCs from patients with
Acknowledgment
This research was supported by Research and Development Program for New Bio-industry Initiatives (2006–2010) of Bio-oriented Technology Research Advancement Institution (BRAIN), Japan.
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