Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume 1691, Issue 1, 1 April 2004, Pages 51-65
Long-term agonist stimulation of IP prostanoid receptor depletes the cognate Gsα protein in membrane domains but does not change the receptor level
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Keywords
IP prostanoid receptor
Gsα protein
Long-term agonist stimulation
Membrane domain
Abbreviations
BPB
bromphenol blue
DIMs
detergent-insoluble or detergent-insensitive membrane domains
DMEM
Dulbecco's modified Eagle's medium
FLAG
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
FhIPR
Flag-tagged form of human IP prostanoid receptor
FhIPR-Gsα
FhIPR-Gsα fusion protein
G proteins
heterotrimeric guanine nucleotide-binding regulatory proteins
Gsα
G protein stimulating adenylyl cyclase activity
Giα
G proteins inhibiting adenylyl cyclase activity in pertussis-toxin sensitive manner
Gqα/G11α
G proteins stimulating phosholipase C in pertussis-toxin independent manner
GPCR
G protein coupled receptor
HEK
human embryonic kidney
GPI
glycosylphosphatidylinositol
Mr
relative molecular weight
PBS
phosphate-buffered saline
PM
plasma membranes
PMSF
phenylmethylsulfonyl fluoride
Na,K-ATPase
sodium plus potassium activated, ouabain dependent adenosine triphosphatase (EC 3.6.1.3)
SLB
solubilisation lysis buffer
TBS
Tris-buffered saline
TRH
thyrotropin releasing hormone
TRH-R
TRH receptor
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