American Journal of Obstetrics and Gynecology
SMFM abstractPoster session II237: Microrna expression in placenta of patients with preeclampsia
Section snippets
Objective
Preeclampsia is associated with altered throphoblastic invasion and misexpression of placental genes. MicroRNAs (miRNAs) are critical posttranscriptional regulators of gene expression. This study was performed using the most comprehensive microaray platform to determine whether preeclampsia is associated with alterations in miRNA expression detected in placenta tissue
Study Design
This is a prospective cohort of hispanic patients seen at BenTaub General Hospital. Severe preeclampsia was defined according to ACOG criteria. Patients with chronic hypertension and superimposed preeclampsia, premature rupture of membranes, multiple gestation, clinical chorioamnionitis, HIV or viral hepatitis infection, stillbirth, or fetal anomalies were excluded. Placenta samples were collected immediately after delivery, placed in RNA-later solution and stored and small RNA extracted.
Results
Microarray comparison using the most comprehensive miRNA array identified 91 misregulated miRNAs. Thirty eight miRNAs were downregulated, and included hsa-miR-451; hsa-let-7g; hsa-miR-411*;hsa-miR47; hsa-miR-572; hsa-miR-1;and hsa-let-7i among others, and 53 were upregulated, and included: hsa-miR-455-5p; hsa-miR-455-3p; hsa-miR-372; hsa-miR-301a; hsa-miR-371-5p; hsa-miR-373; hsa-miR-520c-3p among others.
Conclusion
This different expression patterns of placenta miRNAs identified in this pilot study provide novel targets for further investigation of the pathophysiology of preeclampsia