The Effects of Cadmium on the Fluidity and H+-ATPase Activity of Plasma Membrane from Sunflower and Wheat Roots

doi:10.1016/S0176-1617(11)81418-5

Journal of Plant Physiology

Volume 147, Issue 1, 1995, Pages 87-92

https://doi.org/10.1016/S0176-1617(11)81418-5 Get rights and content

Summary

The effects of cadmium on the H⁺-ATPase activity and on the membrane fluidity were studied in plasma membrane prepared from roots of sunflower and wheat. Plasma membrane purified from the microsomal fraction by phase partitioning showed a decreased ATPase activity by in vivo Cd²⁺ treatment (30 % and 90 % decrease in wheat and sunflower, respectively) and the in vitro application caused an inhibition, as well. The ordering state of the membrane at the C-5 and C-16 levels of the fatty acid chains and at the membrane surface was determined by electron spin resonance (ESR) and by fluorescence spectroscopy, respectively. The presence of Cd²⁺ increased the ordering of the plasma membrane in both in vivo treatments and in vitro experiments. Under Cd²⁺ treatment, a more rigid structure of the PM was found at different depths in the membrane; changes were larger in sunflower than in wheat. The alterations can be the results of direct effects on the membrane constituents (proteins, lipids) or of the modification in lipid composition. Preliminary studies suggest that there are changes caused by Cd²⁺ in the fatty acid composition of the phospholipid fraction in PM, which were different in the case of wheat and sunflower.

References (23)

L.F. De Filippis et al.
Effect of sublethal concentrations of zinc, cadmium and mercury on the photosynthetic carbon reduction cycle of Euglena
J. Plant Physiol.
(1993)
L. Erdei et al.
Effects of tannin and phenolics on the H⁺-ATPase activity in plant plasma membrane
J. Plant Physiol.
(1994)
P. Jost et al.
Lipid spin labels in lecithin multilayers
A study of motion along fatty acid chains. J. Mol. Biol.
(1971)
J.G. Kuhry et al.
Plasma membrane fluidity measurements on living cells by fluorescence anisotropy of trimethylammonium diphenyl hexatriene
Biochim. Biophys. Acta
(1985)
M.A.K. Markwell et al.
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
Anal. Biochem.
(1978)
R. Ros et al.
In vivo and in vitro effects of nickel and cadmium on the plasmalemma ATPase from rice (Oryza sativa L.) shoots and roots
Plant Sci.
(1992)
A. Stroínski et al.
Effects of cadmium on the host pathogen system. IV. Influence of cadmium and Phytophora infestans on membrane permeability of potato tuber
J. Plant Physiol.
(1993)
A. Szabó-Nagy et al.
The effects of iron deficiency on the ATPase and ferricyanide reductase activities of plasma membrane purified by partitioning from sunflower roots
J. Plant Physiol.
(1993)
M. Bartolf et al.
Partial characterization of a cadmium binding protein from the roots of cadmiumtreated tomato
Plant Physiol.
(1980)
A. Bérczi et al.
Comparison of the properties of plasma membrane vesicles purified from wheat roots by phase partitioning and by discontinuous sucrose gradient centrifugation
Physiol. Plant.
(1986)

S.W. Breckle et al.

Ecological geobotany/autecology and ecotoxicology

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In this growing age of population,agriculture plays a significant role by providing food and employment to millions of people. But to meet the growing need of food day by day the demand of fast and quality plant production becomes a must. Fertilization is one of such activities which are people accustomed to do for this purpose from a very long time. But the excessive uses of chemical fertilizers are showing negative influence on the environmental and public health. The paper mainly focuses on how the excessive use of chemical fertilizers are affecting the soil health as well as the water bodies by accumulating heavy metals (HMs) and other chemical elements present in them and the possible remediation measures.In adequate levels, all heavy metals are hazardous. However, some of them e.g., arsenic (As), lead (Pb) and Cadmium (Cd) are of particular relevance due to their environmental concentrations. The paper also provides a comprehensive discussion of the sources, uses, toxicity, and remediation of these particular HMs.
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Journal of Plant Physiology

Summary

References (23)

Effect of sublethal concentrations of zinc, cadmium and mercury on the photosynthetic carbon reduction cycle of Euglena

J. Plant Physiol.

Effects of tannin and phenolics on the H⁺-ATPase activity in plant plasma membrane

J. Plant Physiol.

Lipid spin labels in lecithin multilayers

A study of motion along fatty acid chains. J. Mol. Biol.

Plasma membrane fluidity measurements on living cells by fluorescence anisotropy of trimethylammonium diphenyl hexatriene

Biochim. Biophys. Acta

A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples

Anal. Biochem.

In vivo and in vitro effects of nickel and cadmium on the plasmalemma ATPase from rice (Oryza sativa L.) shoots and roots

Plant Sci.

Effects of cadmium on the host pathogen system. IV. Influence of cadmium and Phytophora infestans on membrane permeability of potato tuber

J. Plant Physiol.

The effects of iron deficiency on the ATPase and ferricyanide reductase activities of plasma membrane purified by partitioning from sunflower roots

J. Plant Physiol.

Partial characterization of a cadmium binding protein from the roots of cadmiumtreated tomato

Plant Physiol.

Comparison of the properties of plasma membrane vesicles purified from wheat roots by phase partitioning and by discontinuous sucrose gradient centrifugation

Physiol. Plant.

Ecological geobotany/autecology and ecotoxicology

Cited by (137)

A critical review on the ecotoxicity of heavy metal on multispecies in global context: A bibliometric analysis

Toxic heavy metals: A bibliographic review of risk assessment, toxicity, and phytoremediation technology

Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata

Effect of uncontrolled fertilization and heavy metal toxicity associated with arsenic(As), lead(Pb) and cadmium (Cd), and possible remediation

Effect of biosorptive removal of cadmium ions from hydroponic solution containing indigenous garlic peel and mercerized garlic peel on lettuce productivity

Cytotoxicity of metal/metalloids’ pollution in plants