ArticlesSpecies Differences in Regional Patterns of 3H-8-OH-DPAT and 3H-Zolpidem Binding in the Rat and Human Brain
Section snippets
Subjects
Male Sprague–Dawley (Charles River, Raleigh, NC) rats weighing 300–400 g were maintained under a 12 L:12 D cycle with continuous access to Purina rat chow and water. Rats were killed by decapitation and brains were frozen with powdered dry ice and stored at −70°C before sectioning.
Human brain regions were dissected at autopsy as authorized by the Chief Medical Examiner, State of North Carolina. Subjects died suddenly from gun shot wounds, stab wounds, vehicular trauma, or sudden cardiac arrest.
3H-8-OH-DPAT Binding in Rat and Human Brain
Discrete and selective labeling of neuroanatomical regions was observed with 3H-8-OH-DPAT in the rat and human brain. For both species, the topography of binding was identical across individual subjects. Although concordance in the neuroanatomical distribution 3H-8-OH-DPAT binding was found in some regions for rats and humans, substantial species differences were also observed.
3H-8-OH-DPAT Binding in Rat and Human Cerebral Cortex
A distinctly different laminar pattern of 3H-8-OH-DPAT binding was observed in human cerebral cortical regions in
Discussion
Similar binding affinities and pharmacological selectivity between rats and humans have been demonstrated with numerous ligands for neurotransmitter receptors, including 3H-OH-DPAT and 3H-zolpidem [see 12, 18, 23, 36, 37, 38. Thus, at a molecular level, the rat appears to be an excellent model system for neuropharmacological research. At a behavioral level also, the rat has proven to be a valuable heuristic model to characterize actions of drugs and to identify compounds that modulate 5-HT1A
Acknowledgements
This work was supported by MH-39144, AA00214, MH-33127, HD-03110, DA09491, and AA09079.
References (49)
- et al.
Expression of c-fos in regions of the basal limbic forebrain following intracerebroventricular corticotropin-releasing factor in unstressed or stressed male rats
Neuroscience
(1992) - et al.
5-HT1A receptor influences on rodent social and agonistic behaviorA review and empirical study
Neurosci. Biobehav. Rev.
(1994) - et al.
Comparative autoradiographic distribution of central (benzodiazepine) modulatory site subtypes with high, intermediate and low affinity for zolpidem and alpidem
Brain Res.
(1993) - et al.
Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain—A combined in situ hybridization/in vitro receptor autoradiographic study
Brain Res.
(1991) - et al.
Pattern and time course of immediate early gene expression in rat brain following acute stress
Neuroscience
(1995) - et al.
Multiple benzodiazepine receptorsNo reason for anxiety
Trends Pharmacol. Sci.
(1992) - et al.
Distribution of 3H-zolpidem binding in relation to mRNA encoding the 1, 2, and 2 subunits of GABAA receptors in rat brain
Neuroscience
(1995) - et al.
Neuroanatomical characterization of the induction of Fos in rat behavioral models of anxiety
Brain Res.
(1996) - et al.
Autoradiographic characterization of 3H-imipramine and 3H-citalopram binding in rat and human brainSpecies differences and relationships to serotonin innervation patterns
Brain Res.
(1992) - et al.
β-Adrenergic receptor distribution in human and rat hippocampal formationmarked species differences
Brain Res.
(1991)