Elsevier

Analytical Biochemistry

Volume 319, Issue 2, 15 August 2003, Pages 335-336
Analytical Biochemistry

Notes & Tips
Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA

https://doi.org/10.1016/S0003-2697(03)00286-0Get rights and content

Section snippets

Acknowledgments

Financial assistance from the Department of Biotechnology, Government of India is acknowledged.

References (4)

There are more references available in the full text version of this article.

Cited by (101)

  • Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites

    2022, Cell Host and Microbe
    Citation Excerpt :

    For the deep mutational scanning, libraries were prepared as biological duplicates by conducting all steps with two independent replicates. The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah, 2003). Oligos were designed to introduce the degenerate codon NNK at the residue of interest to introduce all possible amino acids.

View all citing articles on Scopus
View full text