Short communicationThe MCS1/SSD1/SRK1/SSL1 gene is involved in stable maintenance of the chromosome in yeast
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Cited by (43)
Post-transcriptional control of fungal cell wall synthesis
2022, The Cell SurfaceCitation Excerpt :S. cerevisiae Ssd1 was discovered as a suppressor of deletion of SIT4, a protein phosphatase that regulates the cell cycle (Sutton et al., 1991). Ssd1 also appears as a hit in genetic screens for cell cycle, aneuploidy, temperature stress, and antifungal sensitivity (Wilson et al. 1991; Uesono et al., 1994; Mir et al., 2009; Hose et al. 2020; Schwarzmüller et al. 2014). Ssd1 homologues are required for virulence of diverse ascomycete fungal pathogens (Tanaka et al. 2007; Lee et al. 2010; Thammahong et al. 2019; Tanaka et al. 2009).
Yeast PIAS-type Ull1/Siz1 is composed of SUMO ligase and regulatory domains
2005, Journal of Biological ChemistryCitation Excerpt :Plasmids—Plasmids pT-17 (pTS901CL-ULL1-HA), pT-23 (pTS910CU-ULL1-GFP), pT-32 (pTS901CL-ull1S460C-HA), pT-35 (pGEX-KG-SMT3gg), pT-39 (pGAD-SMT3), pT-60 (pGEX-KG-UBC9), pT-77 (pET21b-ull1ΔC440), and pT-81 (pTS910CU-ull1ΔC440-GFP) were described previously (14, 15, 22). YCUp4-CDC28 contained the 3.5-kb XbaI-SalI fragment carrying CDC28 on YCUp4 vector (31). To construct the plasmid pT-82 (pET21a-ull1-N1), pT-83 (pET21a-ull1-N2), or pT-84 (pET21a-ull1-RING), DNA fragments carrying each amino acid sequence of the ULL1 open reading frame from position 112 to 465, 223 to 465, or 318 to 465, were amplified by PCR, using genomic DNA of W303-1A as template and the following primers, ULL1-ΔN 111-EcoRI-21a (5′-CCGGAATTCACCCCATTGAGTGCTATCAC-3′), ULL1-ΔN222-EcoRI-21a (5′-CCGGAATTCAATAGCAAACACAGGCTATACT-3′), and ULL1-ΔN317-EcoRI-a (5′-CGGAATTCCAACTCCTGGAAAAAGTATTAC-3′), respectively, together with ULL1-ΔC 440-SalI (5′-GCGCGTCGACAGTACCTTTTTCTGGGCTTCT-3′), cut with EcoRI and SalI, and inserted into pET21a vector (Novagen).
Rsp5-Bul1/2 complex is necessary for the HSE-mediated gene expression in budding yeast
2003, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Strains of S. cerevisiae used in this study are listed in Table 1. KA31-2A [18], YAT2-1C [1], and YHY009K [10] were described previously. pHSE-1 plasmid [19] was digested with StuI, introduced into wild-type (KA31-2A), bul1 bul2 (YHK009K), or rsp5-101 (YAT2-1C) cells, and Ura+ transformants integrated with HSE-lacZ were selected to generate YDK004, YDK005, or YDK006, respectively.
Rsp5 WW domains interact directly with the carboxyl-terminal domain of RNA polymerase II
2000, Journal of Biological ChemistryExoribonucleases and their multiple roles in RNA metabolism
2000, Progress in Nucleic Acid Research and Molecular Biology