The types of neuron in spinal dorsal horn which possess neurokinin-1 receptors

doi:10.1016/0306-4522(95)00039-L

Neuroscience

Volume 66, Issue 3, June 1995, Pages 597-608

https://doi.org/10.1016/0306-4522(95)00039-L Get rights and content

Abstract

In order to provide further information about the types of spinal neuron which possess neurokinin-1 receptors, we have carried out pre-embedding immunocytochemistry on sections of rat lumbar spinal cord with an antiserum raised against a synthetic peptide corresponding to part of the sequence of the receptor, and combined this with post-embedding immunocytochemistry to detect GABA and glycine. Numerous neuronal cell bodies showing neurokinin-1 receptor-immunoreactivity were seen in lamina I, laminae III–IV, the lateral spinal nucleus and the area around the central canal. Most of the cells observed in lamina III were small and had relatively restricted dendritic trees which could often not be followed into lamina II, however some larger cells in laminae III and IV had dendrites which extended through lamina II and into lamina I. Cells of the latter type are likely to represent a major target of substance P released from small-diameter primary afferents in the superficial dorsal horn. The great majority (255 out of 283) of spinal neurons which possessed neurokinin-1 receptor-immunoreactivity, including all of those in lamina I, were not GABA- or glycine-immunoreactive, however a few cells in the deep part of the dorsal horn and the lateral spinal nucleus and several cells near the central canal were GABA-immunoreactive, and some of these were also glycine-immunoreactive.

These results suggest that substance P acts through neurokinin-1 receptors mainly on excitatory neurons within the spinal cord.

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The types of neuron in spinal dorsal horn which possess neurokinin-1 receptors

Abstract

Neurosci. Lett.

Neurosci. Lett.

Neuroscience

Neuroscience

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Brain Res.

Molec. Brain Res.

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Neuroscience

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Brain Res.

Brain Res.

Brain Res.

Neurosci. Lett.

Neuroscience

Neuroscience

Neuroscience

Peptides

Neuroscience

Brain Res.

Brain Res.

Different populations of parvalbumin- and calbindin-D28k-immunoreactive neurons contain GABA and accumulate3H-D-aspartate in the dorsal horn of the rat spinal cord

J. comp. Neurol.

The origin, distribution and synaptic relationships of substance P axons in rat spinal cord

J. comp. Neurol.

The neurons of the gelatinosal complex (laminae II and III) of the monkey ( Macaca mulatta): a Golgi study

J. comp. Neurol.

Physiology and morphology of substantia gelatinosa neurons intracellularly stained with horseradish peroxidase

J. comp. Neurol.

The correlation between the distribution of NK1 receptor and the actions of tachykinin agonists in the dorsal horn of the rat indicates that substance P does not have a functional role on substantia gelatinosa (lamina II) neurons

J. Neurosci.

Organization in the Spinal Cord

The cells of origin of the spinothalamic tract of the rat: a quantitative re-examination

Brain Res.

Autoradiographic localization and characterization of spinal cord substance P binding sites: high densities in sensory, autonomic, phrenic, and Onuf's motor nuclei

J. Neurosci.

Different populations of parvalbumin- and calbindin-D28k-immunoreactive neurons contain GABA and accumulate³H-D-aspartate in the dorsal horn of the rat spinal cord

The correlation between the distribution of NK₁ receptor and the actions of tachykinin agonists in the dorsal horn of the rat indicates that substance P does not have a functional role on substantia gelatinosa (lamina II) neurons