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doi:10.1016/0303-7207(92)90087-M 
Copyright © 1992 Published by Elsevier Science Ireland Ltd.
Rapid paper
Identification of a minor Tg mRNA transcript in RNA from normal and goitrous thyroids
Received 24 December 1991;
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Abstract
Polymerase chain reaction (PCR) amplification of nt 4502 to nt 5184 of the thyroglobulin (Tg) mRNA from several patients, with or without elevated serum thyrotropin (TSH), showed a predominant fragment of the expected size (683 bp) and a minor fragment of 512 bp. The sequence of this minor fragment revealed that 171 bp were missing between position 4567 and 4737. It is highly probable that the deleted sequence corresponds to a complete exon, suggesting an alternative splicing as mechanism for the generation of the minor transcript.
Author Keywords: Thyroglobulin gene; Thyroglobulin mRNA; Deletion; Molecular variant; Thyroid, human; Polymerase chain reaction
Abbreviations: Tg, thyroglobulin; TPO, thyroperoxidase; TSH, thyrotropin; PCR, polymerase chain reaction; RT, reverse transcription; nt, nucleotide; kb, 103 bases; bp, base pairs; dNTP, deoxyribonucleotide triphosphate; nt numbering starts at nt 1 of Tg mRNA, amino acid numbering corresponds to that of the mature protein without the leader peptide
