The effect of sodium fluoride at prophylactic and toxic doses on renal structure and function in the isolated perfused rat kidney

doi:10.1016/0300-483X(94)02875-U

Toxicology

Volume 95, Issues 1–3, 6 January 1995, Pages 55-71

https://doi.org/10.1016/0300-483X(94)02875-U Get rights and content

Abstract

To assess the renal effects of fluoride, isolated rat kidneys were perfused in single pass mode for 120 min. Five, 15 and 50, as well as 150, 500 and 1500 μmol NaF were administered 60, 80 and 100 min after starting the perfusion, respectively. Kidneys were perfused with constant pressure (100 mmHg). The perfusate consisted of a substrate supplemented Ringer solution containing hydroxy ethyl starch (HES) to produce isoncotic conditions. Concentrations below 500 μmol/1 NaF did not induce major changes in the main parameters of renal function. Only upon admixture of the highest concentration of 1500 μmol/1 NaF severe changes in renal function could be observed, resulting in complete anuria and a drastic reduction of renal perfusion to 5% of control, associated with a cessation of glomerular filtration. Due to the lack in tubular load, tubular reabsorptive processes inevitably declined to zero. The morphological analysis of kidneys exhibited to 500 μmol/1 NaF revealed the occurrence of vesicular material within the urinary space. These vesicles could electron microscopically be identified as membrane enclosed material of podocytic origin. The interstitium was widened. Upon admixture of 1500 μmol/1 NaF, kidneys responded with a decrease of the interstitial space. Moreover, epithelial cell swelling, hydropic degeneration of all proximal and distal tubular segments, bleb formation and intraluminal casts were observed frequently. Glomerular capillaries were filled with fine precipitates and their endothelium was severely damaged. The results of our studies in the isolated perfused rat kidney (IPRK) model clearly demonstrate a direct dose dependent acute nephrotoxic effect of NaF only for extremely high doses, which, however, may be reached in human cases of severe fluoride intoxication. On the contrary, for low fluoride doses, especially for those concentrations occurring in human plasma upon cariesprophylactic intake of fluorides, no signs of direct acute nephrotoxic action could be observed in the IPRK model.

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The effect of sodium fluoride at prophylactic and toxic doses on renal structure and function in the isolated perfused rat kidney

Abstract

Kidney Int.

Kidney Int.

Kidney Int.

Toxicol. Lett.

Kidney Int.

Caries preventive water fluoridation

Caries Res.

Pathophysiology and pathobiochemistry of acute renal failure

Caries preventive fiuoride tablet Programms

Caries Res.

Kidney function and structure in chronic fluorosis

Br. J. Exp. Pathol.

Selective vulnerability of the medullary thick ascending limb to anoxia in the isolated perfused rat kidney

J. Clin. Invest.

Transport-dependent anoxic cell injury in the isolated perfused rat kidney

Am. J. Pathol.

Substrates induce hypoxic injury to medullary thick ascending limbs of isolated rat kidneys

Am. J. Physiol.

Caries-preventive fiuoride treatment of the individual

Caries Res.

Renal effect of isoflurane

Ma. Tsui. Hsueh. Tsa. Chi.

Nephrotoxicity associated with methoxyflurane anesthesia

Anesthesiology

Fluorine in dental caries

Am. J. Orth. Oral Surg.

Domestic water and dental caries II: A study of 2832 white children, aged 12 to 14 years, of 8 suburban Chicago communities, including Lactobacillus acidophilus studies of 1761 children

Public Health Rep.

Domestic water and dental caries V: Additional studies of the relation of fiuoride domestic waters to dental caries experience in 4425 white children, aged 12 to 14 years, of 13 cities in 4 states

Public Health Rep.

Biophysical basis of glomerular filtration

Fiuoride poisoning and cholinesterases in rats

Acta Pharmacol. Toxicol.

Pharmakokinetics of fiuoride in man and its clinical relevance

J. Dent. Res.

Improved function with amino acids in the isolated perfused kidney

Am. J. Physiol.

Funktion und Morphologie der isolierten Rattenniere nach zellfreier Perfusion mit verschiedenen Plasmaexpandern

Anaesthesist

Beitrag zur experimentellen Knochenfluorose

Int. Arch. Arbeitsmed.

Plasma inorganic fluoride with sevoflurane anesthesia: Correlation with indices of hepatic and renal function

Anesth. Analg.

Fluorosis in cattle

Acute oral toxicity of dentrifice fluorides in rats and mice

J. Dent. Res.

Inorganic and organic fluorine in human blood

Bioverfügbarkeit von Fluorid in Abhängigkeit von der Bindungsform und von Nahrungsbestandteilen

Dtsch. Zahnarztl. Z.

Determination of inorganic fluoride in blood with a fluoride ion selective electrode

Clin. Chem.

Isoflurane sedation for patients undergoing mechanical ventilation: Metabolism to inorganic fluoride and renal effects

Br. J. Anesth.