Trends in Genetics
Technical focusWhole-mount in situ hybridization in the mouse embryo: gene expression in three dimensions
References (36)
EMBO J.
(1988)- et al.
Cell
(1992) Nature
(1989)- et al.
- et al.
Dev. Biol.
(1984) - et al.
- et al.
Chromosoma
(1989) - et al.
Cell
(1991) - et al.
Cell
(1991)
Dev. Biol.
Development
Methods Cell Biol.
Nature
Development
Cell
Curr. Opin. Cell Biol.
Cited by (184)
AmAMP1 from Acropora millepora and damicornin define a family of coral-specific antimicrobial peptides related to the Shk toxins of sea anemones
2021, Developmental and Comparative ImmunologyCitation Excerpt :In brief, material stored at −20 °C in absolute methanol was rehydrated to phosphate buffered saline, pH 7.4 (PBS). It was then transferred to RIPA solution (Rosen and Beddington, 1993) overnight at room temperature with gentle rotation for delipification. Delipification was completed by ethanol dehydration to xylene for several hours, followed by rehydration to PBS, pre-hybridization and hybridization at 55 °C for a minimum of 48 h followed by repeated washes in hybridization wash.
Expression of the neuropeptides RFamide and LWamide during development of the coral Acropora millepora in relation to settlement and metamorphosis
2019, Developmental BiologyCitation Excerpt :In brief, material stored at −20 °C in absolute methanol was rehydrated to PBS. It was then transferred to RIPA solution (Rosen and Beddington, 1993) overnight at room temperature with gentle rotation for delipification. Delipification was completed by ethanol dehydration to xylene for several hours, followed by rehydration to PBS, pre-hybridization and hybridization at 55 °C for a minimum of 48 h followed by repeated washes in hybridization wash.
Di (2-ethylhexyl) phthalate exposure during pregnancy disturbs temporal sex determination regulation in mice offspring
2015, ToxicologyCitation Excerpt :Genital ridges were dissected from fetal mice, and fixed in 4% paraformaldehyde (PFA) overnight (o/n) at 4 °C. WISH with chromogen NBT/BCIP staining was carried out as described elsewhere (Rosen and Beddington, 1993; Schulz et al., 2007; Wei et al., 2011). Antisense and sense probes were synthesized using Digoxigenin RNA labeling mix (Roche) according to the manufacturer's instructions.