Microbial glycolipid production under nitrogen limitation and resting cell conditions

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Abstract

Rhodococcus erythropolis is able to synthesize an anionic trehalose-2,2′,3,4-tetraester during cultivation on n-alkanes. Preconditions for an overproduction are nitrogen limitation, temperature- and pH-shift. The optimum carbon source was technical grade n-C-10, which led to 0.35 g g−1 of glycolipid per n-alkane. Electron microscopical observations showed that n-C-14,15 (technical grade) grown cells contained numerous lipid inclusions in contrast to n-C-10 (technical grade) grown cells. Nocardia corynebacteroides synthesizes a novel pentasaccharide lipid and as side products small amounts of trehalose-corynomycolates. Optimum precursors for overproduction are n-alkanes from n-tetradecane to n-hexadecane with yields in the range of 0.17 g g−1 of glycolipid per carbon source.

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Presented at the 2nd International Symposium on Overproduction of Microbial Products, held in Česke Budějovice, Czechoslovakia, 3–9 July, 1988.

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