S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP

doi:10.1016/0092-8674(89)90059-7

Cell

Volume 57, Issue 7, 30 June 1989, Pages 1223-1236

https://doi.org/10.1016/0092-8674(89)90059-7 Get rights and content

Abstract

The endoplasmic reticulum (ER) of mammalian cells contains a 78 kd protein (BiP) that is believed to assist in the folding of secretory and transmembrane proteins. We have used a cDNA encoding mouse BiP to isolate the homologous gene from S. cerevisiae, which encodes a sequence of 682 amino acids, 431 of which are identical to mouse BiP. Like its mammalian counterpart, yeast BiP is encoded by an HSP70-like gene whose transcription is stimulated by the presence of unfolded polypeptides in the ER. The gene encoding yeast BiP is essential for cell growth and, unexpectedly, is identical to the recently cloned KAR2 gene. Expression of mammalian BiP in S. cerevisiae can complement a mutant allele of KAR2 that is temperature sensitive for growth and nonconditionally defective for karyogamy. These results suggest that deficiencies in BiP may cause generalized failure of protein folding in the ER, leading to pleiotropic effects on cellular metabolism.

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Cell

ArticleS. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP

Abstract

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Meth. Enzymol.

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Exp. Cell Res.

Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat-shock genes

Science

mRNA cap-binding protein: cloning of the gene encoding protein synthesis initiator eIF-4E from Saccharomyces cerevisiae

Mol. Cell. Biol.

Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum

J. Cell Biol.

Translocation in yeast and mammalian cells: not all signal sequences are functionally equivalent

J. Cell Biol.

Sec11 is required for signal peptide processing and yeast cell growth

J. Cell Biol.

Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas

J. Cell Biol.

Copper metallothionein of yeast, structure of the gene, and regulation of expression

Assembly of multisubunit membrane proteins

Rat gene encoding the 78-kDa glucose-regulated protein GRP78: its regulatory sequences and the effect of protein glycosylation on its expression

70K heat shock related proteins stimulate protein translocation into microsomes

Nature

Isolation of a yeast centromere and construction of small circular chromosomes

Nature

A mutant of Saccharomyces cerevisiae defective for nuclear fusion

Assembly of influenza hemagglutinin trimers and its role in intracellular transport

J. Cell Biol.

The heat shock response

Crit. Rev. Biochem.

Mutations in cognate genes of Saccharomyces cerevisiae hsp70 result in reduced growth rates at low temperatures

Mol. Cell. Biol.

Expression of the S. cerevisiae Hsp70 multigene family

SSC1, a member of the 70-kDa heat shock protein multigene family of Saccharomyces cerevisiae, is essential for growth

Article
S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP