Elsevier

Methods in Enzymology

Volume 224, 1993, Pages 294-309
Methods in Enzymology

[21] Use of randomly amplified polymorphic DNA markers in comparative genome studies

https://doi.org/10.1016/0076-6879(93)24022-MGet rights and content

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Detection of genetic variation is essential to a wide range of comparative genetic research endeavors. The speed and accuracy of comparative genetic research often depend on the methodology for detecting variation. The advent of allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analysis of DNA have made it possible to detect many polymorphisms in most organisms at the protein or DNA level These genetic polymorphisms are crucial in examining the many different fields. Specifically, polymorphisms can aid in the determination of relatedness of groups of taxa, in the analysis of parentage in domestic and wild animal species, in the identification of individuals for captive breeding programs in endangered species, in the comparison of wild and cultivated plants, and in the estimation of levels of inbreeding or outbreeding in populations. This chapter discusses the use of randomly amplified polymorphic DNA markers in comparative genome studies.

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