Elsevier

Thrombosis Research

Volume 72, Issue 3, 1 November 1993, Pages 175-182
Thrombosis Research

Paper
Aspirin fails to inhibit platelet aggregation in sheep

https://doi.org/10.1016/0049-3848(93)90184-PGet rights and content

Abstract

This is the first report in which aspirin (ASA) has failed to inhibit aggregation of mammalian platelets. Preincubation of citrated sheep platelet rich plasma with a final concentration of 500 μM ASA for 3 minutes at 37°C did not inhibit aggregation induced by either arachidonic acid (AA; 1.6 mM), ADP (2.5 μM), collagen (5.6 μg/ml) or thrombin (0.04U). Instead, ASA potentiated the aggregation response produced by these agents except AA. Platelet aggregation that was reversible with ADP became irreversible after adding ASA. The inhibitory properties of ASA was confirmed with human platelets, challenged with AA, ADP, adrenalin and collagen. These findings suggest that sheep platelets have an ASA resistant cyclo oxygenase and may be able to aggregate by a pathway that is independent of arachidonic acid.

References (26)

  • Z. Sinakos et al.

    Platelet aggregation in mammalians (human, rat, rabbit, guinea pig, horse, dog) A comparative study

    Thrombos Diathes Haemorrh

    (1967)
  • D.C.B. Mills

    Platelet aggregation and platelet nucleotide concentration in different species

  • D.P. Thomas et al.

    Release of adenine nucleotides and platelet factor 4 from platelets of man and 4 other species

    J Lab Clin Med

    (1970)
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