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3.2 kb was isolated by RNA differential display applied to developing mouse skin. 
doi:10.1016/0014-5793(90)81184-P 
Copyright © 1990 Published by Elsevier Science B.V. All rights reserved.
Research letters
Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA A variant lacking the transmembrane domain
Ichiro Kato, Hideto Yonekura, Hiroshi Yamamoto and Hiroshi Okamoto
Received 12 June 1990.
Abstract
We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
Author Keywords: Peptide α-amidating enzyme; Functional expression; Secretory form; Transmembrane domain; Alternative splicing; mRNA
Abbreviations: kbp, kilobase pairs; bp, base pairs; Hepes, 4(2-hydroxyethyl)-1-piperazineethane sulfonic acid
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