ScienceDirect - FEBS Letters : Cloning and expression of cDNA encoding human basic fibroblast growth factor

Volume 213, Issue 1, 9 March 1987, Pages 189-194

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doi:10.1016/0014-5793(87)81489-8

Research letters

Cloning and expression of cDNA encoding human basic fibroblast growth factor

Tsutomu Kurokawa ^,^a, Reiko Sasada^a, Makoto Iwane^a and Koichi Igarashi^a

^aBiotechnology Laboratories of Central Research Division, Takeda Chemical Industries, Ltd, Yodogawaku, Osaka 532, Japan

Received 29 December 1986.

Available online 2 November 2001.

Abstract

A cDNA encoding human basic fibroblast growth factor (bFGF) was isolated from a human foreskin fibroblast cDNA library. The cDNA, 4 kilobases in size, had a coding sequence, 5' and 3' untranslated regions, and a poly(A) chain. Isolation of additional cDNA clones that had a short 3' untranslated region suggested the presence of multiple mRNA forms. By Northern blot analysis, at least five bFGF mRNA species were detected in cultured fibroblast cells. Transfection of the cDNA to COS cells resulted in the detection of mitogenic activity in the culture medium of the transfected cells, suggesting that a part of the synthesized protein might be secreted from cells despite its unusual short signal sequence.

Keywords: Fibroblast growth factor; cDNA; mRNA; Secretion; (COS cell)

References

[1]D. Gospodarowicz, P. Rudland, J. Lindstrom and K. Benirschke, Adv. Metab. Disorders 8 (1975), pp. 302–335.

[2]F. Esch, A. Baird, N. Ling, N. Ueno, F. Hill, L. Deneroy, R. Klepper, D. Gospodarowicz, P. Bohlen and R. Guillemin, Proc. Natl. Acad. Sci. USA 82 (1985), pp. 6507–6511. View Record in Scopus | Cited By in Scopus (0)

[3]D. Gospodarowicz In: R.J. Ford and A.L. Maizel, Editors, Mediators in Cell Growth and Differentiation, Raven, New York (1984), pp. 109–134. View Record in Scopus | Cited By in Scopus (1)

[4]D. Gospodarowicz, A.L. Mescher and C.R. Birdwell, Natl. Cancer Inst. Monogr. 48 (1978), pp. 109–130. View Record in Scopus | Cited By in Scopus (8)

[5]F. Esch, A. Baird, N. Ling, N. Ueno, F. Hill, L. Denoroy, R. Klepper, D. Gospodarowicz, P. Bohlen and R. Guillemin, Proc. Natl. Acad. Sci. USA 82 (1985), pp. 6507–6511. View Record in Scopus | Cited By in Scopus (120)

[6]J.A. Abraham, A. Mergia, J.L. Whang, A. Tumolo, J. Friedman, K.A. Hjerrild, D. Gospodarowicz and J.C. Fiddes, Science 233 (1986), pp. 545–548. View Record in Scopus | Cited By in Scopus (243)

[7]G. Gimenez-Gallego, G. Conn, V.B. Hatcher and K.A. Thomas, Biochem. Biophys. Res. Commun. 135 (1986), pp. 541–548. Abstract | Article |

PDF (505 K) | View Record in Scopus | Cited By in Scopus (19)

[8]H. Okayama and P. Berg, Mol. Cell. Biol. 3 (1983), pp. 280–289. View Record in Scopus | Cited By in Scopus (113)

[9]U. Gubler and B.J. Hoffman, Gene 25 (1983), pp. 263–269. Abstract |

PDF (8669 K) | View Record in Scopus | Cited By in Scopus (656)

[10]T. Maniatis, E.F. Firitsch and J. Sambrook, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).

[11]F. Sanger, S. Nicklen and A.R. Coulson, Proc. Natl. Acad. Sci. USA 74 (1977), pp. 5463–5467. View Record in Scopus | Cited By in Scopus (20586)

[12]J.M. Chirgwin, A.E. Przybyla, R.J. MacDonald and W.J. Rutter, Biochemistry 18 (1979), pp. 5294–5299. View Record in Scopus | Cited By in Scopus (5610)

[13]H. Lehrach, D. Diamond, J.M. Wozney and H. Boedtker, Biochemistry 16 (1977), pp. 4743–4751. View Record in Scopus | Cited By in Scopus (560)

[14]Y. Gluzman, Cell 23 (1981), pp. 175–182. Abstract | Article |

PDF (2829 K) | View Record in Scopus | Cited By in Scopus (543)

[15]F.L. Graham and der Eb A.J. Van, Virology 54 (1973), pp. 536–539. Abstract | Article |

PDF (1510 K) | View Record in Scopus | Cited By in Scopus (195)

[16]T. Maciag, J. Cerundole, S. Ilsley, P.R. Kelley and R. Forand, Proc. Natl. Acad. Sci. USA 76 (1979), pp. 5674–5678. View Record in Scopus | Cited By in Scopus (221)

[17]M. Kozak, Nucleic Acids Res. 12 (1984), pp. 857–872. View Record in Scopus | Cited By in Scopus (930)

[18]N.J. Proudfoot and G.G. Brownlee, Nature 263 (1976), pp. 211–214. View Record in Scopus | Cited By in Scopus (368)

[19]E.M. Alderman, R.R. Lobb, J.W. Fett, J.F. Riordan, J.L. Bethune and B.L. Vallee, Biochemistry 24 (1985), pp. 7866–7871. View Record in Scopus | Cited By in Scopus (1)

[20]Heijne G. Von, Eur. J. Biochem. 133 (1983), pp. 17–21.

[21]P.E. Auron, A.C. Webb, L.J. Rosenwasser, S.F. Mucci, A. Rich, S.M. Wolff and C.A. Dinarello, Proc. Natl. Acad. Sci. USA 81 (1984), pp. 7907–7911. View Record in Scopus | Cited By in Scopus (127)

[22]R. Lobb, J. Sasse, R. Sullivan, Y. Shing, P. D'Amore, J. Jacobs and Klagsbrun, J. Biol. Chem. 261 (1986), pp. 1924–1928. View Record in Scopus | Cited By in Scopus (33)

[23]J.A. Abraham et al., EMBO J. 5 (1986), pp. 2523–2528. View Record in Scopus | Cited By in Scopus (156)

Correspondence address: T. Kurokawa, Biotechnology Laboratories of Central Research Division, Takeda Chemical Industries, Ltd, Yodogawaku, Osaka 532, Japan.

Volume 213, Issue 1, 9 March 1987, Pages 189-194

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