Alcian blue staining of proteoglycans in polyacrylamide gels using the “Critical electrolyte concentration” approach
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Cited by (52)
First proteomic analyses of the dorsal and ventral parts of the Sepia officinalis cuttlebone
2017, Journal of ProteomicsA minimal molecular toolkit for mineral deposition? Biochemistry and proteomics of the test matrix of adult specimens of the sea urchin Paracentrotus lividus
2016, Journal of ProteomicsCitation Excerpt :This new fraction was referred as the LS-AIM (Laemmli-soluble, acetic acid insoluble). The gels were stained with Bio-Safe Coomassie G250 (CBB, Bio-Rad), Silver Nitrate [19], Alcian Blue [20] and carbocyanine [21] (also called “Stains-all”). The ASM and AIM were analyzed on a 2D-gel Protean IEF cell (Bio-Rad), according to the manufacturer's instructions.
The test skeletal matrix of the black sea urchin Arbacia lixula
2015, Comparative Biochemistry and Physiology - Part D: Genomics and ProteomicsCitation Excerpt :Samples were loaded on gels, which were subsequently run for 15 min at 100 V, then for about 1 h at 150 V. For visualization of proteins, gels were stained with Bio-Safe Coomassie G250 (Bio-Rad, ref. 161-0787) and with silver nitrate according to the protocol of Morrissey (1981). Alcian blue staining protocol was used to stain polyanionic macromolecules, including acidic proteoglycans (Lev and Spicer, 1964; Wall and Gyi, 1988). PAS staining was applied to stain saccharide moieties (Zacharius et al., 1969).
Changes in the connective tissue sheath of Wistar rat nerve with aging
2014, Annals of AnatomyCitation Excerpt :To stop the process, solution II was decanted and solution III (50% (v/v) methanol, 12% (v/v) acetic acid) was immediately added (Blum et al., 1987). Others gels were submitted to Alcian blue method for GAGs detection, according Wall and Gyi (1988). The total extract (50 μg of proteins) of the nerves was used for metalloproteinase types 2 (MMP-2) and 9 (MMP-9) detection and evaluation of activity.
The shell matrix of the pulmonate land snail Helix aspersa maxima
2012, Comparative Biochemistry and Physiology - B Biochemistry and Molecular BiologyLocalization of Bacillus thuringiensis Cry1A toxin-binding molecules in gypsy moth larval gut sections using fluorescence microscopy
2011, Journal of Invertebrate Pathology