Abstract
The gastroprotective effects of Jacaranda puberula Cham., Bignoniaceae, a tree native to the Brazilian Atlantic Forest, were evaluated using two models of acute ulceration in mice: acidified ethanol- and non-steroidal anti-inflammatory-induced ulceration. The mice were divided into five groups and treated orally with vehicle, carbenoxolone, or hydroalcoholic extract obtained from the tested plant (3, 30, or 300 mg/kg). Results showed that the doses of 300 mg/kg decreased both acidified ethanol- and indomethacin-induced ulceration by 89% and 33%, respectively, compared to the vehicle (p < 0.001). These results were similarly to those obtained for the carbenoxolone-treated group. Gastroprotection was confirmed by histological analysis. The gastroprotective mechanisms involve reductions in inflammatory and oxidative stress markers, including a reduction in myeloperoxidase activity, along with increases in glutathione levels and superoxide dismutase activity. Jacaranone and verbascoside were identified as the active components.
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Funding
This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) and Unochapecó [modality Art. 170 and 171 - FUMDES] UFPR, FINEP, CNPq, and Fundação Araucária.
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All authors contributed substantially to the work reported. WARJ and PZS conceived and designed the experiments and wrote the paper. AJP, DM, and MECZ performed the chemical isolation, and MFCS and AB identified the compounds using NMR techniques. GL, CDV, DBG, BMMO, AMS, and CSC performed the in vivo experiments and the biochemical analyses. JAL, SFA, and LMS analyzed the data and collaborated to write the paper.
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The authors declare that they have no conflict of interest.
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The experiments were approved by the Institutional Animal Ethics Committee of Universidade Comunitária da Região de Chapecó (protocol number 020/17).
All the international standards and the ethical guidelines on animal welfare were strictly followed throughout the experimentation.
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Zanotelli, P., Locateli, G., Vecchia, C.D. et al. Gastroprotective Potential of the Hydroalcoholic Extract from Jacaranda puberula in Mice. Rev. Bras. Farmacogn. 30, 838–843 (2020). https://doi.org/10.1007/s43450-020-00117-2
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DOI: https://doi.org/10.1007/s43450-020-00117-2