Abstract
Purpose
The aim of this study was to find the optimal detection method for cucumber powdery mildew and improve the identification efficiency.
Methods
Image segmentation technology was used to extract spot images and grade classification of powdery mildew. The relationship between powdery mildew spot and spectral reflectance and intensity was studied. The powdery mildew detection model was established by using qualitative analysis and quantitative prediction methods combined with greenness (a*) indices of cucumber leaves.
Results
There were strong positive correlations between greenness and spectrum in some characteristic bands. Through the extraction of disease spot images and disease classification, it was found that the higher the disease grade of leaves was, the higher the spectral reflectivity and fluorescence intensity. In the quantitative prediction model, the R2 of the NIR spectrum was improved (0.8742) after MSC and SPA, and the effect of the fluorescence spectrum model was not ideal. In the qualitative discriminant model, KNN and ensemble subspace discriminant were obtained for two kinds of spectra, and the identification accuracy of the qualitative model was 97.5% after verification.
Conclusion
An NIR spectral model can be used for the quantitative prediction of cucumber powdery mildew. The qualitative discriminant model had high accuracy for cucumber powdery mildew.
Similar content being viewed by others
References
Araújo, M. C. U., Saldanha, T. C. B., Galvão, R. K. H., Yoneyama, T., & Visani, V. (2001). The successive projections algorithm for variable selection in spectroscopic multicomponent analysis. Chemometrics and Intelligent Laboratory Systems, 57(2), 65–73. https://doi.org/10.1016/S0169-7439(01)00119-8
Bai, X. B., Yu, J. S., Fu, Z. T., Zhang, L. X., & Li, X. X. (2019). Segmentation and detection of cucumber powdery mildew based on visible spectrum and image processing. Spectroscopy and Spectral Analysis, 39(11), 3592–3598. https://doi.org/10.3964/j.issn.1000-0593(2019)11-3592-07
Guan, H., Zhang, C. L., & Zhang, C. Y. (2010). Grading method of cucumber leaf spot disease based on image processing. Journal of Agricultural Mechanization Research, 32(3), 94–97. https://doi.org/10.3969/j.issn.1003-188X.2010.03.025
Li, X. X., Zhu, C. G., Bai, X. B., Mao, F. H., Fu, Z. T., & Zhang, L. X. (2019). Recognition method of cucumber leaves diseases based on visual spectrum and support vector machine. Spectroscopy and Spectral Analysis, 39(7), 2250–2256. https://doi.org/10.3964/j.issn.1000-0593(2019)07-2250-07
Li, H. B., He, G. Z., & Guo, Q. T. (2015). Spectral similarity retrieval method of organic matter based on Pearson correlation coefficient. Chemical Analysis and Metrology, 24(03), 33–37. https://doi.org/10.3969/j.issn.1008-6145.2015.03.009
Liu, Y. D., & Niu, H. M. (2011). Small sample KNN classification algorithm based on k-nearest neighbor graph. Computer Engineering, 37(9), 198–200. https://doi.org/10.3969/j.issn.1000-3428.2011.09.069
Liu, Z. B., & Wang, S. T. (2011). Improved linear discriminant analysis algorithm. Computer Applications, 31(1), 250–253. https://doi.org/10.3724/SP.J.1087.2011.00250
Pan, C. H., Xiao, D. Q., Lin, T. Y., & Wang, C. T. (2018). Classification and identification of main vegetable pests in South China based on SVM and regional growth algorithm. Transactions of the Chinese Society of Agricultural Engineering, 34(8), 192–199. https://doi.org/10.11975/j.issn.1002-6819.2018.08.025
Sankaran, S., & Ehsani, R. (2013). Detection of huanglongbing-infected citrus leaves using statistical models with a fluorescence sensor. Applied Spectroscopy, 67(4), 463–469. https://doi.org/10.1366/12-06790
Włodarska, K., Khmelinskii, I., & Sikorska, E. (2018). Evaluation of quality parameters of apple juices using near-infrared spectroscopy and chemometrics. Journal of Spectroscopy, 2018, 1–8. https://doi.org/10.1155/2018/5191283
Wu, D., Liu, W. F., Hu, S., Hu, L. Z., & Hu, J. H. (2017). Color image segmentation using K-mean clustering based on lab space. Electronic Science and Technology, 30(10), 29–32. https://doi.org/10.16180/j.cnki.issn1007-7820.2017.10.009
Yi, T. Y. (2014). Powdery mildew of vegetables. Hunan Agriculture, 10, 20–21. https://doi.org/10.3969/j.issn.1005-362X.2014.10.024
Yu, W. J., Wang, C. X., Qiao, L., Wang, S. L., & He, X. G. (2020). Construction of PLSR prediction model for chromaticity of Jingyuan yellow beef based on hyperspectral imaging technology. Zhejiang Journal of Agricultural Sciences, 32(3), 527–533. https://doi.org/10.3969/j.issn.1004-1524.2020.03.19
Zhang, P., Zhu, Y. Q., Wang, L. L., & Zhou, S. J. (2017). Recognition of cucumber leaf powdery mildew based on machine vision. Chinese Agricultural Science Bulletin, 33(21), 134–137. https://doi.org/10.11924/j.issn.1000-6850.casb16090110
Zhang, H. Y., Liu, Y., Ma, L. M., Yuan, J. S., Ju, H. J., & Wei, T. J. (2017b). Comparison and application of decision tree algorithm. North China Electric Power Technology, 6, 42–47. https://doi.org/10.16308/j.cnki.issn1003-9171.2017.06.008
Zhang, N., Yang, G. J., Pan, Y. C., Yang, X. D., Chen, L. P., & Zhao, C. J. (2020). A review of advanced technologies and development for hyperspectral-based plant disease detection in the past three decades. Remote Sensing, 12(19), 3188. https://doi.org/10.3390/rs12193188
Funding
This study was funded by the LiaoNing Revitalization Talents Program (XLYC2007043) and the Scientific Research Fund Project of Liaoning Province ( LJKZZ20220087).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare no competing interests.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Xu, J.T., Zhang, Z., Guo, Y.H. et al. Detection of Cucumber Powdery Mildew Based on Spectral and Image Information. J. Biosyst. Eng. 48, 115–122 (2023). https://doi.org/10.1007/s42853-023-00178-w
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42853-023-00178-w