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Occurrence of antimicrobial-resistant Staphylococcus aureus in a Brazilian veterinary hospital environment

  • Veterinary Microbiology - Research Paper
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Abstract

Antimicrobial resistance is a threat to public health. The emergence of antibiotic-resistant Staphylococcus aureus represents a priority for the implementation of preventive measures. The objective was to isolate S. aureus in humans, animals, and animal health care environment, and to characterize the genotypic and phenotypic profile of antimicrobial resistance in these isolates. We isolated S. aureus from staff, animals, and environment of a veterinary hospital, and identified their antimicrobial resistance profiles. Samples were collected from 20 humans, 13 animals, 14 surfaces, 8 mobile phones, and 7 veterinarians’ stethoscopes by using sterile swabs. S. aureus was isolated by culturing on mannitol salt agar and preliminary identification was done by Gram staining and catalase test. Subsequently, a polymerase chain reaction was performed for species confirmation and investigating their antimicrobial-resistant genotypic profiles. Phenotypic profiles of resistant isolates were determined using the disk-diffusion technique. Ten S. aureus isolates were recovered from 5/20 humans (25%), it was also recovered from 2/13 animals (15.38%), including 1 dog and 1 cat, and from 1/14 of surfaces (7.14%). The oxacillin-susceptible mecA-positive Staphylococcus aureus phenotype was identified in a feline. Most of the isolates carried at least two resistance genes of different antimicrobial classes, with 90% (9/10) presenting the gene blaZ, with 10% (1/10) presenting the gene mecA, 20% (2/10) presenting tet38, 10% (1/10) presenting tetM, 90% (9/10) presenting norA, 50% (5/10) presenting norC, 10% (1/10) presenting ermA, and 60% (6/10) presenting ermB. In antibiograms, resistance to penicillin was identified in all the isolates, resistance to erythromycin was identified in 80% (8/10), and all the isolate’s resistance to erythromycin presented erythromycin-induced resistance to clindamycin. Antimicrobial resistance in the veterinary hospital requires attention due to the risk of interspecies transmission, gene transfer between bacteria that colonize companion animals and humans and, can make antimicrobial therapy difficult.

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Acknowledgements

We appreciate the contribution of the members of the Veterinary Hospital of the Department of Veterinary Medicine of the Federal Rural University of Pernambuco – HOVET/DMV/UFRPE. We thank the veterinarians and tutors. The authors are grateful to UFRPE, the Postgraduate Program in Animal Bioscience (PPGBA). The authors are grateful to the National Council for Scientific and Technological Development (CNPq) (financial code: 400921/2019-7) and the Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES), for the fellowship and financial support that made this research possible.

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Authors and Affiliations

  1. Department of Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil

    Denny Parente de Sá Barreto Maia Leite, Iago Carvalho Barbosa, Renato Amorim da Silva, Pollyanne Raysa Fernandes, Atzel Candido Acosta Abad & Rinaldo Aparecido Mota

  2. Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil

    Denny Parente de Sá Barreto Maia Leite, Iago Carvalho Barbosa, Renato Amorim da Silva, Pollyanne Raysa Fernandes, Atzel Candido Acosta Abad & Tatiana Souza Porto

  3. Department of Preventive Veterinary Medicine and Animal Production, School of Veterinary Medicine and Zootechnics, Federal University of Bahia (UFBA), Salvador, Bahia, Brazil

    José Givanildo da Silva

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  1. Denny Parente de Sá Barreto Maia Leite
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Correspondence to Rinaldo Aparecido Mota or Tatiana Souza Porto.

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Ethics approval

All experimental procedures were in accordance with the ethics principles accepted by the Ethics Committee for the Use of Animals of the Federal Rural University of Pernambuco (UFRPE), license number 1466270721, as well as by the Plataforma Brazil, license number 46827221.7.0000.9547.

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Leite, D.P., Barbosa, I.C., da Silva, R.A. et al. Occurrence of antimicrobial-resistant Staphylococcus aureus in a Brazilian veterinary hospital environment. Braz J Microbiol 54, 2393–2401 (2023). https://doi.org/10.1007/s42770-023-01035-w

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