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Molecular characterization of indigenous entomopathogenic fungus Beauveria bassiana, isolate and its virulence to Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae)

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Abstract

A fungal culture was isolated with a view to evaluate its potentiality for an effective pest management programme. The fungal culture was characterized at molecular level by using rDNA ITS universal primer. The fungal culture was identified as Beauveria bassiana (Bals.) Vuill. and the ~ 550 bp rDNA-ITS sequence was submitted to the Gene bank and obtained accession number as KR855715. The biological parameters of KR855715 were compared with MTCC strains of Beauveria bassiana (Bals.) Vuill and evaluate its efficacy against sixth instar of Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae), a major stored grain pest of rice under laboratory condition at temperature 30 ± 1 °C and relative humidity 85–90 %. The biological parameters and efficacy of all these strains were tested at different concentrations viz.., 1 × 107to 1 × 109 conidia/ml. Among these strains, KR855715 showed the highest conidial density (8.13 × 107 conidia/ml) at concentration of 1 × 107 conidia/ml. However, the germination (83.68 %), and mortality (60.00 %) was found to be highest at concentration 1 × 109 conidia/ml.

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References

  • Abdulhai M, Bonhssini MEI, Jamal M, Trissi AN, Sayyadi Z, Skinner M, Parker BL (2010) Beauveria bassiana characterization and efficacy vs. Sunn Pest, Euryaster integriceps Putan (Hemiptera): Seutelleridae). Pak J Biol Sci 13:105–108

    Article  Google Scholar 

  • Akbari S, Safavi SA, Ghosta Y (2014) Efficacy of Beauveria bassiana (Blas.) Vuill. against cabbage aphid Brevicoryne brassicae L. (Hem.: Aphididae) in laboratory condition. Arch Phytopathol Plant Protect 47:1454–1458. https://doi.org/10.1080/03235408.2013.845972

    Article  Google Scholar 

  • Anonymous (2019) Agricultural statistics at a glance. Directorate of Economics and Statistics, Govt of India, New Delhi

  • Attallah AG, Nivien AS, Abd-El-Aal SK (2014) Molecular characterization of Beauvaria sp. with Inter-Simple Sequence Repeat (ISSR) and RAPD Markers. Int J Pharm Tech Res 6:1407–1415

    CAS  Google Scholar 

  • Bashir NH, Nazir T, Nasir M, Majeed MZ, Hanan A, Sagheer M, Hasan M (2018) In vitro Entomopathogenic Efficacy of Beauveria bassiana (Ascomycota: Hypocreales) Against Corcyra cephalonica (Lepidoptera: Pyralidae) and Tribolium castaneum (Coleoptera: Tenebrionidae). J Entomol 15:56–61

    Article  Google Scholar 

  • Campbell RK, Anderson TE, Semel M, Roberts DW (1985) Management of the Colorado potato beetle using the entomogenous fungus Beauveria bassiana. Am Potato J 62:29–37

    Article  Google Scholar 

  • Dannon HF, Dannon AE, Douro-Kpindou OK, Zinsou AV, Houndete AT, Toffa-Mehinto J, Elegbede IATM, Olou BD, Tamo M (2020) Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. J Cotton Res 3:24. https://doi.org/10.1186/s42397-020-00061-5

    Article  Google Scholar 

  • Das P, Hazarika LK, Bora DS, Puzari KC, Dutta P (2011) Effect of temperature on radial growth, sporulation, germination, colony forming unit and biomass production of different strains of Beauveria bassiana Vuill. (Bals.). Pestol 35:50–55

    Google Scholar 

  • Das P, Hazarika LK, Bora D, Puzari KC, Dutta P (2012) Mass production of Beauveria bassiana (Bals.) Vuill. for the management of rice hispa, Dicladispa armigera (Olivier). J Biol Control 26:347–350

    Google Scholar 

  • Das K, Das P, Kalita S, Borkataki S, Hazarika S (2016) Study on duration of instars and morphomatrics of Corcyra cephalonica (Stainton). Res Crops 17:395–397. https://doi.org/10.5958/2348-7542.2016.00065.6

    Article  Google Scholar 

  • Das P, Hazarika LK, Kaita S, Roy S (2019) Current state of fungal antagonists with special emphasis on Indian Scenario. In: Khan MA, Ahmed W (Eds.) Microbes for Sustainable Insect Pest Management, Sustainability in Plant and Crop Protection. Springer Nature, Cham, 2019 365

  • Devi KU, Padmavathi J, Sharma HC, Seetharama N (2001) Laboratory evaluation of the virulence of Beauveria bassiana isolates to the sorghum shoot borer Chilo partellus Swinhoe (Lepidoptera: Pyralidae) and their characterization by RAPD-PCR. World J Microbiol Biotechnol 17:131–137

    Article  CAS  Google Scholar 

  • Dhar S, Jindal V, Jariyal M, Gupta VK (2019) Molecular characterization of new isolates of the entomopathogenic fungus Beauveria bassiana and their efficacy against the tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Egypt J Biol Pest Control 29:1–9

    Article  Google Scholar 

  • Dhawan M (2015) Bio-efficacy of Beauveria bassiana against cabbage caterpillar Pieris brassicae. MSc.Thesis, Punjab Agricultural University

  • Dutta P, Puzari KC, Hazarika LK, Das P (2012) Natural occurrence of entomogenous fungi and its pathogneicity to insects of North East India. Pestology XXXVI:37–39

    Google Scholar 

  • Fransisco EA, Mochi DA, Correia ACB, Monteiro AC (2006) Influence of culture media in viability test of conidia of entomopathogenic fungi. Crop Protect 36:1–3

    Google Scholar 

  • Gonzalez AR, Mayo S, Lopez OG (2016) Inhibitory activity of Beauveria bassiana and Trichoderma spp. on the insect pests Xylotrechus arvicola (Coleoptera: Cerambycidae) and Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae). Environ Monit Assess 189:12. https://doi.org/10.1007/s10661-016-5719-z

    Article  CAS  Google Scholar 

  • Hazarika LK, Puzari KC (1997) Field efficacy of white muscardine fungus, Beauveria bassiana. Indian J Agril Sci 67:463–465

    Google Scholar 

  • Hazarika LK, Puzari KC, Dutta P (2005) Beauveria bassiana in rice hispa management in Assam. Biocontrol News Inf 2:106–108

    Google Scholar 

  • Imoulan A, Hussain M, Kirk PM, El Meziane A, Yao YJ (2017) Entomopathogenic fungus Beauveria: host specificity, ecology and significance of morpho-molecular characterization in accurate taxonomic classification. J Asia Pac Entomol 20:1204–1212

    Article  Google Scholar 

  • Kaur S, Thakur A, Rajput M (2014) A laboratory assessment of the potential of Beauveria bassiana (Balsamo) Vuillemin as a biocontrol agent of Corcyra cephalonica Stainton (Lepidoptera: Pyralidae). J Stored Product Res 59:185–189

    Article  Google Scholar 

  • Kaushik H, Dutta P, Kalita J (2015) Metarhizium anisopliae a potential entomopathogen: Its persistence and viability in the organic Tea ecosystems of Assam, India. Int J Agric 126:376–379

    Google Scholar 

  • Khan MA, Ahmad W (2015) The management of Spodopteran pests using fungal pathogens. In: Sree KS, Varma A (eds) Biocontrol of lepidopteran pests. Springer International Publishing, Basel, pp 123–160

    Chapter  Google Scholar 

  • Kuberappa GC, Jayaramaiah M (1987) Influence of temperature and humidity on the growth and development of the fungus Beauveria bassiana (Bal.) Vuill. A strain on the silkworm, Bombyx mori L, Mysore. J Agric Sci 21:184–188

    Google Scholar 

  • Li Z, Alves SB, Roberts DW, Fan M, Delalibera I Jr, Tang J, Rangel DE (2010) Biological control of insects in Brazil and China: History, current programs and reasons for their successes using entomopathogenic fungi. Biocontrol Sci Technol 20:117–136

    Article  Google Scholar 

  • Meena HR, Meena A, Kumar A, Meena AK, Chauhan SK, Meena BM (2017) Impact of Abiotic Factors on the Growth and Development of Corcyra cephalonica Stainton in Stored Maize. Int J Curr Microbiol App Sci 6:1599–1608

    Article  Google Scholar 

  • Menge AK, Naik KV, Jalgaonkar VN, Golvankar GM (2018) Bionomics of rice moth, Corcyra cephalonica (st.) on groundnut variety TG – 37. J Entomol Zool Stud 6:36–38

    Google Scholar 

  • Mishra S, Kumar P, Malik A (2013) Evaluation of Beauveria Bassiana spore compatibility with surfactants. Int J Biol Biomol Agric Fd Biotechnol Eng 7:7–11

    Google Scholar 

  • Moore D, Prior C (1996) Mycoinsecticides. In: Upadhyay RK, Mukerjee KG, Rajak RL (eds) IPM system in agriculture. Biocontrol in emerging biotechnology. Aditya Books Private Ltd., New Delhi, pp 25–56

    Google Scholar 

  • Moore D, Douro-Kpindou OK, Jenkins NE, Lomer CJ (1996) Effect of moisture content and temperature on storage of Metarhizium flavoviridae conidia. Biocontrol Sci Technol 6:51–61

    Article  Google Scholar 

  • Mudoi A, Das P, Hazarika LK (2018) Beauveria bassiana (Bals.) Vuill. strains pathogenic to Periplaneta americana (L.). J Ento Zool Stud 6:903–907

    Google Scholar 

  • Mudoi A, Das P, Hazarika LK, Das K, Roy S (2020) Variations in hemocyte profile induced by Beauveria bassiana (Bals.) Vuill. in Periplaneta americana (L.) (Blattodea: Ectobiidae). Int J Trop Insect Sci 40:81–91

    Article  Google Scholar 

  • Mwamburi L, Laing MD, Miller RM (2015) Effect of surfactants and temperature on germination and vegetative growth of Beauveria bassiana. Braz J Microbiol 46:67–74. https://doi.org/10.1590/S1517-838246120131077

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nanjundappa H, Niranjana SR, Prakash HS, Shetty HS (2001) Beauveria bassiana -A potential mycopesticide for the efficient control of Coffee berry borer, Hypothenemus hampei (Ferrari) in india. Biocontrol SciTechnol 11:251–260. https://doi.org/10.1080/09583150120035675

    Article  Google Scholar 

  • Parker BL, Skinner M, Costa SD, Gouili S, Rreid W, Bouhssni ME (2003) Entomopathogenic fungi of Eurygaster integriceps puton (Hemiptera: Scutelleridae) collection and characterization for development. J Biol Control 27:260–272

    Article  Google Scholar 

  • Parveen A, Khataniar L, Goswami G, Hazarika DJ, Das P, Gautom T, Barooah M, Boro RC (2017) A study on the diversity and habitat specificity of macrofungi of assam. India Int J Curr Microbiol App Sci 6:275–297

    Article  Google Scholar 

  • Pegu J, Dutta P, Puzari KC, Das A (2016) Bioefficcay of Metarhizium anisopliae on Aphis craccivora Koch. Indian J Entomol 78:353–355. https://doi.org/10.5958/0974-8172.2016.00091.2

    Article  Google Scholar 

  • Pillai MG, Dayanandan S, Joy B (2017) Impact of four plant powders on the development of the rice moth, Corcyra cephalonica Stainton in rice grain. Int J Recent Sci Res 8:17179–17182

    Article  Google Scholar 

  • Puzari KC, Hazarika LK (1992) Entomopathogenic fungi from North East India. Indian Phytopath 45:35–38

    Google Scholar 

  • Puzari KC, Hazarika LK (1994) Pathogenicity of Beauveria bassiana (Bals.) Vuill, on developmental stages of rice Hispa, Dicladispa Armigera (Olivier). J Biol Ctrl 8:133–135

    Google Scholar 

  • Puzari KC, Hazarika LK, Deka N (1994) Pathogenicity of Beauveria bassiana on rice hispa (Dicladispa armigera). Indian J Agric Sci 64:123–125

    Google Scholar 

  • Puzari KC, Dutta P, Hazarika LK, Hazarika GN, Kaushik H (2015) Beauveria bassina - A potential entomopathogen against rice hispa. Int J Appl Biol Pharm Technol 6:94–99

    Google Scholar 

  • Rao PS, Perraju A, Rao KBH (1980) Effect of fortification of natural rearing media with Casein, Cholesterol and Glucose on Corcyra cephalonica (Stainton): I Larval growth. Indian J Entomol 42:218–222

    Google Scholar 

  • Rashmi MA (2009) Biology of rice moth, Corcyra cephalonica Stainton on foxtail millet. Karnataka J Agric Sci 22:674–675

    Google Scholar 

  • Rosa WDL, Alatorre R, Trujillo J, Barrera JF (1997) Virulence of Beauveria bassiana (Deuteromycetes) strains against the Coffee Berry Borer (Coleoptera: Scolytidae). J Econ Entomol 90:1534–1538. https://doi.org/10.1093/jee/90.6.1534

    Article  Google Scholar 

  • Senthamizhlselvan P, Alice J, Sjeetha RP, Jeyalakshmi C (2010) Growth, sporulation and biomass production of native entomopathogenic fungal isolates on a suitable medium. J Biopest 3:466–469

    Google Scholar 

  • Smith SM, Moore D, Karanja LW, Chandi EA (1999) Formulation of vegetable fat pellets with pheromone and Beauveria bassiana to control the larger grain borer, Prostephanus truncates (Horn). Pestic Sci 55:711–718

    Article  CAS  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197

  • Trivedi A, Nayak N, Kumar J (2018) Recent advances and review on use of botanicals from medicinal and aromatic plants in stored grain pest management. J Entomol Zool Stud 6:295–300

    Google Scholar 

  • Vivekanandhan P, Kavitha T, Karthi S, Senthil-Nathan S, Shivakumar MS (2018) Toxicity of Beauveria bassiana-28 Mycelial Extracts on Larvae of Culex quinquefasciatus Mosquito (Diptera: Culicidae). Int J Environ Res Public Health 15:440. https://doi.org/10.3390/ijerph15030440

    Article  CAS  PubMed Central  Google Scholar 

  • Vivekanandhan P, Bedini S, Shivakumar MS (2020) Isolation and identification of entomopathogenic fungus from Eastern Ghats of South Indian forest soil and their efficacy as biopesticide for mosquito control. Parasitol Int 76:102099

    Article  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols, A Guide to Methods and Applications. Academic, San Diego, pp 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1

  • Wraight S, Ramos ME (2017) Effects of inoculation method on efficacy of wettable powder and oil dispersion formulations of Beauveria bassiana against Colorado potato beetle larvae under low-humidity conditions. Biocontrol Sci Technol 27:348–363. https://doi.org/10.1080/09583157.2017.1291904

    Article  Google Scholar 

  • Yuan HG, Wu SY, Lei ZR, Rondon SI, Gao YL (2018) Sub-lethal effects of Beauveria bassiana (Balsamo) on field populations of the potato tuberworm Phthorimaea operculella Zeller in China. J Integr Agric 17:911–918. https://doi.org/10.1016/S2095-3119(17)61898-7

    Article  Google Scholar 

  • Zimmermann G (2007) Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Sci Technol 17:553–596

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Acknowledgements

Authors are thankful to the Head, Department of Entomology and Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat for providing the necessary facilities.

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Authors and Affiliations

  1. Department of Entomology, Assam Agricultural University, Jorhat, 785013, Assam, India

    Karishma Das, Purnima Das & Abhilisa Mudoi

  2. Department of Agricultural Biotechnology, Assam Agricultural University, 785013, Jorhat, Assam, India

    Assma Parveen & Robin Chandra Boro

  3. Tocklai Tea Research Institute, Tea Research Association, Jorhat, 785008, Assam, India

    Somnath Roy

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  1. Karishma Das
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Correspondence to Purnima Das.

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Das, K., Das, P., Parveen, A. et al. Molecular characterization of indigenous entomopathogenic fungus Beauveria bassiana, isolate and its virulence to Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae). Int J Trop Insect Sci 41, 2443–2451 (2021). https://doi.org/10.1007/s42690-020-00421-3

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