Abstract
Abstract
Bio-diesel is a fuel produced by transesterification reaction of oil or fat in the presence of a catalyst. It is an alternate renewable source for petrol and diesel, which helps in reducing greenhouse gases emissions. Chemical catalysts such as sodium hydroxide, potassium hydroxide, sulfuric acid are used in the conventional methods of biodiesel production. This review focusses on application of lipase enzyme in transesterification of different types of oils such as: soybean, sunflower, rapeseed, palm oil, and waste cooking oil. Worldwide production of biodiesel via lipase enzyme and its economic as well as future aspects are also discussed. Various researchers have used free lipase in biodiesel production however, some lipases are also utilized as immobilized form. There are number of fungal and bacterial species that have been used to synthesize lipases, some of the microbial species are as follows: Aspergillus niger, Burkholderia cepacia, Pseudomonas aeruginosa, Staphylococcus epidermidis etc. Solid-state fermentation and broth culture of fungus is suitable for crude lipase production. Immobilized lipase plays very significant role in transesterification process. Lipases enzymes act as a good catalyst therefore, its production and utilization may be a better alternative of chemical catalysts.
Graphic abstract



Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Abbas H (2002) Isolation and characterization of an extracellular lipase from Mucor stain isolated from palm fruit. Enzyme Micro Technol 31:968–978
Abed KA, Gad MS, El Morsi AK, Sayed MM, Elyazeed SA (2019) Effect of biodiesel fuels on diesel engine emissions. Egypt J Pet 28(2):183–188
Aguieiras E, Oliveira EC, Castro AM, Freire DMG (2014) Biodiesel production from Acrocomia aculeata acid oil by (enzyme/enzyme) hydroesterification process: use of vegetable lipase and fermented solid as low-cost biocatalysts. Fuel 135:315–321
Ajala OE, Aberuagba F, Odetoye TE, Ajala AM (2015) Biodiesel: sustainable energy replacement to petroleum-based diesel fuel—a review. Chem Bio Eng Rev 3:145–156
Amini Z, Ong HC, Harrison MD, Kusumo F, Mazaheri H, Ilham Z (2017) Biodiesel production by lipase-catalyzed transesterification of Ocimum basilicum L. (sweet basil) seed oil. Energy Convers Manag 132:82–90
Angelo T, Silva RD, Cabral H (2014) Concomitant production of peptidases and lipases by fungus using agro-industrial residue in solid-state fermentation. Int J Curr Microbiol Appl Sci 3(5):810–823
Arumugam A, Ponnusami V (2017) Production of biodiesel by enzymatic transesterification of waste sardine oil and evaluation of its engine performance. Heliyon 3(12):1–18
Avhad MR, Marchetti JM (2019) Uses of enzymes for biodiesel production. Advanced bioprocessing for alternative fuels, biobased chemicals, and bioproducts. Woodhead Publishing, Sawston, pp 135–152
Balaji V, Ebenezer P (2008) Optimization of extracellular lipase production in Colletotrichum Gloeosporioides by solid state fermentation. Indian J Sci Technol 1(7):1–8
Bartha-Vari JH, Moisa ME, Bencze LC, Irimie FD, Paizs C, Toșa MI (2020) Efficient biodiesel production catalyzed by nanobioconjugate of lipase from Pseudomonas fluorescens. Molecules 25(3):651
Benjamin S, Pandey A (2001) Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation. Braz Arch Biol Technol 44(2):213–221
Budzaki S, Sundaram S, Tisma M, Hessel V (2019) Cost analysis of oil cake-to-biodiesel production in packed-bed micro-flow reactors with immobilized lipases. J Biosci Bioeng 128(1):98–102
Bueso F, Moreno L, Cedeno M, Manzanarez K (2015) Lipase-catalyzed biodiesel production and quality with Jatropha curcas oil: exploring its potential for Central America. J Biol Eng 9(12):1–7. https://doi.org/10.1186/s13036-015-0009-9
Burkert JFM, Maugeri F, Rodrigues MI (2004) Optimization of extracellular lipase production by Geotrichum sp. using factorial design. Bioresour Technol 91(1):77–84
Chakraborthy K, Rajamani P (2009) Purification and biochemical characterization of an extracellular lipase from Pseudomonas fluorescens MTCC 2421. J Agricul Food Chem 57(9):3858–3866
Chen HC, Ju HY, Wu TT, Liu YC, Lee CC, Chang C, Chung YL, Shieh CJ (2010) Continuous production of lipase-catalyzed biodiesel in a packed-bed reactor: optimization and enzyme reuse study. J Biomed Biotechnol 2011:1–6
Colin VL, Baigori MD, Pera LM (2011) Mycelium -bound Lipase production from Aspergillus niger MYA 135, and its potential application for the transesterification of ethanol. J Basic Microbiol 51:236–242
Costa TM, Harmann KL, Roman MG, Valle RCS, Tavares LBB (2017) Lipase production by Aspergillus niger grown in different agro-industrial waste by solid state fermentation. J Braz Chem Eng 34:419–427
Demirbas A (2008) Biodiesel production from Tall oil. Energy Sourc Part A 30(20):1896–1902
Demirbas A (2009) Progress and recent trends in biodiesel fuels. Energy Convers Manag 50(1):14–34
Desikan S, Kannan R, Narayanan K, Kumar DJM, Kalachelvan PT (2013) Application of immobilized lipase enzyme for the production of biodiesel from waste cooking oil. Asian J Biol Sci 6(7):322–330
Duarte SH, Hernández GL, Canet A, Benaiges MD, Maugeri F, Valero F (2015) Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase. Bioresour Technol 183:175–180
Edupuganti S, Parcha L, Mangamoori LN (2017) Purification and characterization of extracellular lipase from Staphylococcus epidermidis (MTCC 10656). J Appl Pharm Sci 7(1):57–63
Fleuri LF, Nonelli P, Delgado CHO, Piyetta MR, Pereira MS, Arcuri MD, Capoville BL (2014) Biochemical characterization and application of lipases produced by Aspergillus sp. on solid-state fermentation using three substrates. Int J Food Sci Technol 49(2):2585–2591
Foukis A, Gkini OA, Stergiou PY, Sakkas VA, Dim A, Boura K, Koutinas A, Papamichael EM (2017) Sustainable production of a new generation biofuel by lipase-catalyzed esterification of fatty acids from liquid industrial waste biomass. Bioresor Technol 238:122–128
Freitas L, Patrica CM, Ros D, Julio C, Santos Heizir F, Castro D (2009) An integrated approach to produce biodiesel and monoglycerides by enzymatic interestification of babassu oil (Orbinya sp). Process Biochem 44(10):1068–1074
Galeano JD, Mitchell DA, Krieger N (2017) Biodiesel production by solvent-free ethanolysis of palm oil catalyzed by fermented solids containing lipases of Burkholderia contaminans. Biochem Eng J 127:77–86
Hanaki H, Portugal-Pereira J (2018) Effect of biofuel production on greenhouse gas emission reduction. In: Takeuchi K, Shiroyama H, Saito O, Matsuura M (eds) Biodiesel and Sustainability science for sustainable societies. Springer, Tokyo, pp 53–71
Harsha DN, Mahesh DC, Kumar S, Farooq MZ (2015) Study of properties and use of lipid biofuels with Environmental impacts for production of low-cost fuel. Int J Sci Eng Res 6(1):218–222
Huang D, Zhao H, Lin L (2015) Biodiesel an alternative to conventional Fuel. Energy Procedia 16:1874–1885
Iftikhar T, Niaz M, Zia MA, Haq I (2010) Production of extracellular lipases by Rhizopus oligosporus in a stirred fermenter. Brazilian J Microbiol 41:1124–1132
Jegannathan KR, Abang S (2008) Production of biodiesel using immobilized Lipase—a critical review. Crit Rev Biotechnol 28(4):253–264
Jin Z, Hang SY, Zhang L, Zheng SP, Wang Y, Lin W (2013) Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media. Bioresour Technol 130:102–109
Kareem SO, Falokun EI, Balogun SA, Akinloye OA, Omeike SO (2017) Enzymatic biodiesel production from palm oil and palm kernel oil using free lipase. Egypt J Pet 26(3):635–642
Karmee SK (2017) Lipase catalyzed synthesis of fatty acid methyl esters from crude Pongamia Oil. Energy Sources, Part A 37:536–542
Kaur BD, Taggar MS, Sharma P, Kaur P (2017) Screening of bacterial strains for lipase production and its application in biodiesel synthesis. Int J Chem Stu 5:230–236
Khanahmadi S, Yusof F, Ong HC, Amid A, Shah H (2016) Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: an optimized process. J Biotechnol 231:96–105
Kumar DS, Roy S (2014) Fungal lipase production by solid state fermentation—an overview. J Anal Bioanal Tech 6:1–10
Kumar V, Joseph B, Pramod W, Mani RA, Jahan F (2011) Cold active lipase catalyzed production of biodiesel from Jatrospha oil in a solvent free system. J Chem Pharm Res 3:226–233
Kumar D, Das T, Giri BS, Rene ER, Verma B (2019) Biodiesel production from hybrid non-edible oil using bio-support beads immobilized with lipase from Pseudomonas cepacia. Fuel 255:115801
Kumari V, Shah S, Gupta M (2007) Preparation of biodiesel by lipase-catalyzed transesterification of high free fatty acid containing oil from Madhuca indica. Energy Fuels 21:368–372
Lam MK, Lee KT, Mohamed AR (2010) Homogeneous, heterogeneous and enzymatic catalysis for transesterification of high free fatty acid oil (waste cooking oil) to biodiesel: a review. Biotechnol Adv 28:500–518
Li K, Fan Y, He Y, Zeng L, Han X, Yan Y (2017) Burkholderia cepacia lipase immobilized on hetero functional magnetic nanoparticles and its application in biodiesel synthesis. Sci Rep 7(1):1–17
Lu J, Deng L, Zhao R, Zhang R, Wang F, Tan T (2010) Pretreatment of immobilized Candida sp. 99–125 lipase to improve its methanol tolerance for biodiesel production. J Mol Catal B Enzym 62:15–18
Ma Y, Liu Y (2019) Biodiesel production: status and perspectives. Biofuels: alternative feedstocks and conversion processes for the production of liquid and gaseous biofuels. Academic Press, New York, pp 503–522
Madras G, Kolluru C, Kumar R (2004) Synthesis of biodiesel in supercritical fluids. Fuel 83:2029–2033
Mahanta N, Gupta A, Khare SK (2007) Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Bioresour Technol 99:1729–1735
Manurung R, Hasibuan R, Bangun TN, Wulandari R, Sari BI (2016) Transesterification process of palm oil using novozym 435 in choline chloride (CHCL) to produce biodiesel. J Eng Appl Sci 11:9962–9969
Mehrasbi MR, Mohammadi J, Peyda M, Mohammadi M (2017) Covalent immobilization of Candida antarctica lipase on core-shell magnetic nanoparticles for production of biodiesel from waste cooking oil. Renew Energy 101:593–602
Mounguengui RWM, Barea B, Villeneuve P, Blin J (2013) Are plant lipases a promising alternative to catalyze transesterification for biodiesel production? Prog Energy Combust Sci 39:441–556
Mukherjee J, Gupta MN (2016) Lipase coated clusters of iron oxide nanoparticles for biodiesel synthesis in a solvent free medium. Bioresour Technol 209:166–171
Musa IM (2016) The effects of alcohol to oil molar ratios and the type of alcohol on biodiesel production using transesterification process. Egypt J Pet 25:21–31
Naqvi SH, Dahot MU, Ali A, Khan MY, Rafiq M (2011) Production and characterization of extracellular lipase secreted by Mucor geophillus. Afr J Biotechnol 10:19598–19606
Narsimharao K, Brown DR, Lee AF, Newman AD, Siril PF, Tavener SJ (2007) Structure–activity relations in Cs-doped heteropolyacid catalysts for biodiesel production. J Catal 248:226–234
Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R (2015) Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius. BioMed Res Int. https://doi.org/10.1155/2015/281934
Nematian T, Shakeri A, Salehi Z, Saboury AA (2020) Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from Chlorella vulgaris bio-oil. Biotechnol Biofuels 13(1):1–15
Okoro OV, Sun Z, Birch J (2019) Lipases for Biofuel Production. In: Encyclopedia of Food Chemistry, pp 150–157. https://doi.org/10.1016/B978-0-08-100596-5.21650-8
Paques FW, Macedo GA (2006) Lipases de latex vegetais: properties and industrial applications. Quim Nova 29:93–99
Pitol LO, Finkler ATJ, Dias GS, Machado AS, Zanin GM, Mitchell AD, Krieger N (2017) Optimization studies to develop a low-cost medium for production of the lipases of Rhizopus microsporus by solid-state fermentation and scale-up of the process to a pilot packed-bed bioreactor. Process Biochem 62:37–47
Qin HE, Xu Y, Teng Y, Wang D (2008) Biodiesel production catalyzed by whole-cell lipase from Rhizopus chinensis. Chin J Catal 29:41–46
Quayson E, Amoah J, Rachmadona N, Hama S, Yoshida A, Kondo A, Ogino C (2020) Biodiesel-mediated biodiesel production: a recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils. Fuel Proc Technol 199:106278
Rathi P, Goswami VK (2002) Statistical medium optimization and production of a hyperthermostable lipase from Burkholderia cepacia in a bioreactor. J Appl Microbiol 93:930–936
Rodrigues J, Canet A, Rivera I, Osorio NM, Sandoval G, Valero F, Dias FS (2016a) Biodiesel production from crude jatropha oil catalyzed by non-commercial immobilized heterologous Rhizopus oryzae and Carica papaya lipases. Bioresour Technol 213:88–95
Rodrigues J, Perrier V, Lecomte J, Dubreucq E (2016b) Dias FS. Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium. Bioresor Technol 218:1224–1229
Rouhany M, Montogomery H (2019) Global biodiesel production, the state of art and impact on climate change. Springer, Cham, pp 1–14
Sandoval G, Casas-Godoy L, Bonet-Ragel K, Rodrigues J, Ferreira-Dias S, Valero F (2017) Enzyme-catalyzed production of biodiesel as alternative to chemical-catalyzed processes: advantages and constraints. Curre Biochem Eng 4(2):109–141
Sankaran R, Show PL, Chang JS (2016) Biodiesel production using immobilized lipase: feasibility and challenges. Biofuels Bioprod Bioref 10(6):896–916
Sebastian J, Muraleedharan C, Santhiagu A (2017) Enzyme catalyzed biodiesel production from rubber seed oil containing high free fatty acid. Int J Green Energy 14:687–693
Selvakumar P, Sivashanmugam P (2017) Optimization of lipase production from organic solid waste by anaerobic digestion and its application in biodiesel production. Fuel Process Technol 165:1–8
Shah S, Sharma S, Gupta MN (2004) Biodiesel preparation by lipase-catalyzed transesterification of jatropha oil. Energy Fuels 18:154–159
Shahedi M, Yousefi M, Habibi Z, Mohammadi M, As’habi MA (2019) Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology. Renew Energy 141:847–857
Shahir VK, Jawahar CP, Suresh PR (2015) Comparative study of diesel and biodiesel on CI engine with emphasis to emissions—a review. Renew Sust Energ Rev 45:686–697
Sirajuddin M, Tariq M, Ali S (2015) Organotin (IV) carboxylates as an effective catalyst for the conversion of corn oil into biodiesel. J Organomet Chem 779:30–38
Suci M, Arbianti R, Hermansyah H (2018) Lipase production from Bacillus subtilis with submerged fermentation using waste cooking oil. Earth Environ Sci 105:1–6
Suwanno S, Rakkan T, Yunu T, Paichid N, Kimtun P, Prasertsan P, Sangkharak K (2017) The production of biodiesel using residual oil from palm oil mill effluent and crude lipase from oil palm fruit as an alternative substrate and catalyst. Fuel 195:82–87
Tamalampudi S, Talukder MR, Hama S, Numata T, Kondo A, Fukuda H (2008) Enzymatic production of biodiesel from Jatropha oil: a comparative study of immobilized-whole cell and commercial lipases as a biocatalyst. Biochem Eng J 39:185–189
Tran HL, Ryu YJ, Seong DH, Lim SM, Lee CG (2013) An effective acid catalyst for biodiesel production from impure raw feedstocks. Biotechnol Bioprocess Eng 18:242–247
Utami TS, Hariyani I, Alamsyah G, Hemansyah H (2017) Production of dry extract extracellular lipase from Aspergillus niger by solid state fermentation method to catalyze biodiesel synthesis. Energy Procedia 236:41–46
Venkatessagowada B, Pangupaty E, Barbosa-Dekker AM, Dekker RFH (2017) The purification of lipase from Lasiodiplodia theobromae and their immobilization and use for Biodiesel production from Coconut oil. Appl Biochem Biotechnol 185:619–640
Vishnupriya B, Sundaramoorthi C, Kalaivani M, Selvam K (2010) Production of lipase from Streptomyces griseus and evaluation of Bio-parameters. Int J Chem Tech Res 20102:1380–1383
Wancura JHC, Tres MV, Jahn SL, de Oliveira JV (2019) Lipases in liquid formulation for biodiesel production: current status and challenges. Biotechnol Appl Biochem. https://doi.org/10.1002/bab.1835
Wani SP, Girish C, Kanwar LS, Srinivasa R, Raghvendra G, Susanna P, Pavani M (2012) Carbon sequestration and rehabilitation through Jatropha curcas, (L.) plantation in degraded land. Agric Ecosyst Environ 161:112–120
World Bank (2008) The growth report: strategies for sustained growth and inclusive development
Xie W, Huang M (2018) Immobilization of Candida rugosa lipase onto graphene oxide Fe3O4 nanocomposite: characterization and application for biodiesel production. Energy Convers Manag 159:42–53
Yan J, Zheng X, Du L, Shengving L (2014) Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts. Biotechnol Biofuels 7:2–8
Yucel S, Terziaglu P, Ozcimen D (2012) Lipase applications in biodiesel production. In: Zhen F (ed) Biodiesel—feedstocks, production and applications. IntechOpen, pp 209-249, DOI: 10.5772/52662
Yucel Y, Demir C, Dizge N, Keskinler B (2014) Methods for Lipase Immobilization and Their Use for Biodiesel Production from Vegetable Oil. Energy Res 36:1203–1211
Zhong L, Feng Y, Wang G, Wang Z, Bilal M, Lv H, Cui J (2020) Production and use of immobilized lipases in/on nanomaterials: a review from the waste to biodiesel production. Int J Biol Macromol 152:207–222
Zouaoui B, Bouziane A, Ghalem BR (2012) Isolation, purification and properties of lipase from Pseudomonas aeruginosa. Afr J Biotechnol 1:12415–12421
Zuhair SA (2007) Production of biodiesel: possibilities and challenges. Biofuels Bioprod Bioref 1:57–66
Acknowledgements
One of us (Chandra Bhan) is thankful to the University Grant Commission (UGC), New Delhi India for providing UGC non-net fellowship.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Bhan, C., Singh, J. Role of microbial lipases in transesterification process for biodiesel production. Environmental Sustainability 3, 257–266 (2020). https://doi.org/10.1007/s42398-020-00119-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42398-020-00119-9