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Monitoring progression of the die back pathogen Phomopsis azadirachtae in neem seedlings

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Abstract

In the present study, the progression of Phomopsis azadirachtae infection in neem was evaluated using two methods namely, ergosterol estimation and qRT-PCR method. Using artificially infected neem seedlings the disease progression was tabulated at specific intervals by observing the disease symptoms, like the characteristic lesions and drying. The ergosterol method estimated a mycelial mass ranging from 0.0008–0.093 µg/g, the qRT-PCR method estimated mycelial mass ranging from 0.0008–0.099 µg/g. Both the methods indicated the progression of infection within the neem tree. In conclusion, the pathogen was exponentially high around the 20th day post infection, suggesting optimal establishment within the neem plants.

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Acknowledgements

All authors thank the Principal SJCE, Mysore for his support and encouragement throughout the execution of this work. The authors also thank the President and Secretary of SDME Society, Ujire for their encouragement.

Funding

Prithvi S. Shirahatti thank The Department of Science and Technology, Science and Engineering Research Board, New Delhi, India for awarding the Research fellowship.

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Authors and Affiliations

  1. Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, Mysore, Karnataka, 570 006, India

    Prithvi S. Shirahatti, S. Nanjunda Swamy & M. N. Nagendra Prasad

  2. Division of Biotechnology and Bioinformatics, Department of Water & Health Sciences - Faculty of Life sciences, JSS Academy of Higher Education and Research (JSS AHER), SS Nagar, Mysuru, Karnataka, 570 015, India

    Ramith Ramu

  3. Microbiology Division, Defence Food Research Laboratory, Siddharth Nagar, Mysore, Karnataka, 570 011, India

    Joseph Jeyabalaji Kingston

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  1. Prithvi S. Shirahatti
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  2. Ramith Ramu
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  3. S. Nanjunda Swamy
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  4. Joseph Jeyabalaji Kingston
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  5. M. N. Nagendra Prasad
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Corresponding author

Correspondence to M. N. Nagendra Prasad.

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Shirahatti, P.S., Ramu, R., Swamy, S.N. et al. Monitoring progression of the die back pathogen Phomopsis azadirachtae in neem seedlings. Indian Phytopathology 72, 139–146 (2019). https://doi.org/10.1007/s42360-018-0099-7

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  • DOI: https://doi.org/10.1007/s42360-018-0099-7

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