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Properties of Collagen/Sodium Alginate Hydrogels for Bioprinting of Skin Models

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Abstract

3D printing technology has great potential for the reconstruction of human skin. However, the reconstructed skin has some differences from natural skin, largely because the hydrogel used does not have the appropriate biological and physical properties to allow healing and regeneration. This study examines the swelling, degradability, microstructure and biological properties of Collagen/Sodium Alginate (Col/SA) hydrogels of differing compositions for the purposes of skin printing. Increasing the content of sodium alginate causes the hydrogel to exhibit stronger mechanical and swelling properties, a faster degradation ratio, smaller pore size, and less favorable biological properties. An optimal 1% collagen hydrogel was used to print bi-layer skin in which fibroblasts and keratinocytes showed improved spreading and proliferation as compared to other developed formulations. The Col/SA hydrogels presented suitable tunability and properties to be used as a bioink for bioprinting of skin aiming at finding applications as 3D models for wound healing research.

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Data Availability Statement

The data and materials that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This research was funded by the National Key R&D Program of China (2018YFE0207900), and People's Liberation Army (BWS17J036, 18-163-13-ZT-003-011-01) and the National Natural Science Foundation of China (51835010 and 51375371), and Xi’an Science and Technology Plan Project (21ZCZZHXJS-QCY6-0012). Shaanxi Science and Technology Project (2022KXJ-147). Thanks to Shi Changquan and Yang Chuncheng of Shaanxi Ketao-AM Technology Co., Ltd. for their technical support for printing equipment.

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Authors and Affiliations

  1. State Key Laboratory for Manufacturing System Engineering, Xi’an Jiaotong University, Xi’an, 710049, China

    Tian Jiao, Qin Lian, Weilong Lian, Yonghui Wang & Dichen Li

  2. Shaanxi Ketao-AM Technology Co., Ltd., Xi’an, 710100, China

    Qin Lian

  3. 3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Barco, 4805-017, Guimarães, Portugal

    Rui L. Reis & Joaquim Miguel Oliveira

  4. ICVS/3B’s–PT Government Associate Laboratory, Barco, 4805-017, Guimarães, Portugal

    Rui L. Reis & Joaquim Miguel Oliveira

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  1. Tian Jiao
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Jiao, T., Lian, Q., Lian, W. et al. Properties of Collagen/Sodium Alginate Hydrogels for Bioprinting of Skin Models. J Bionic Eng 20, 105–118 (2023). https://doi.org/10.1007/s42235-022-00251-8

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  • DOI: https://doi.org/10.1007/s42235-022-00251-8

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