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18F-PBR06 PET/CT imaging of inflammation and differentiation of lung cancer in mice

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Abstract

The present study explored the 18-kDa translocator protein radioligand 18F-PBR06 as a PET imaging biomarker for diagnosis of inflammation and compared it with 18F-FDG for differentiation of inflammation and lung tumors in animals. 18F-PBR06 was synthesized with an average decay-corrected radiochemical yield of 30–40% (end of synthesis, EOS), and the radiochemical purity was greater than 99%. The inflammation-to-blood ratio of 18F-PBR06 (3.53 ± 0.26) was higher than the tumor-to-blood ratio (1.77 ± 0.35) (P < 0.001). The inflammation-to-muscle ratio of 18F-PBR06 (2.33 ± 0.64) was also higher than the tumor-to-muscle ratio (1.45 ± 0.14) (P = 0.036). Micro-PET/CT images showed high uptake of 18F-FDG in both inflamed muscles and lung tumor tissues. However, 18F-PBR06 uptake in inflamed muscles remained higher than that in the lung tumor tissues, following 90 min of dynamic Micro-PET/CT imaging. Further, macrophages in the inflammatory regions showed a higher fluorescence signal than in lung tumor tissues. Results of the study confirmed that 18F-PBR06 PET/CT imaging allowed for diagnosis of inflammation. Moreover, 18F-PBR06 uptake in the inflammatory regions was significantly higher than in lung tumor tissues, suggesting that 18F-PBR06 PET/CT imaging has potential to differentiate between peripheral lung cancer and inflammation nodules.

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Authors and Affiliations

  1. Shanghai Institute of Medical Imaging, Shanghai, 200032, China

    He Zhang, Hui Tan, Wu-Jian Mao, Jun Zhou, Zhe-Quan Fu, Yan Hu, Jie Xiao, Qing-Yu Lin, Hong-Cheng Shi & Deng-Feng Cheng

  2. Department of Nuclear Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    He Zhang, Hui Tan, Wu-Jian Mao, Jun Zhou, Zhe-Quan Fu, Yan Hu, Jie Xiao, Qing-Yu Lin, Hong-Cheng Shi & Deng-Feng Cheng

  3. Institute of Nuclear Medicine, Fudan University, Shanghai, 200032, China

    He Zhang, Hui Tan, Wu-Jian Mao, Jun Zhou, Zhe-Quan Fu, Yan Hu, Jie Xiao, Qing-Yu Lin, Hong-Cheng Shi & Deng-Feng Cheng

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  1. He Zhang
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Correspondence to Hong-Cheng Shi or Deng-Feng Cheng.

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This work was funded in part by the National Natural Science Foundation of China (Nos. 11875114, 81471706, and 81871407), Science and Technology Commission of Shanghai Municipality (No. 16410722700), and sponsored by the Shanghai Sailing Program (No. 17YF1417400).

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Zhang, H., Tan, H., Mao, WJ. et al. 18F-PBR06 PET/CT imaging of inflammation and differentiation of lung cancer in mice. NUCL SCI TECH 30, 83 (2019). https://doi.org/10.1007/s41365-019-0597-z

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