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Analyte-responsive fluorescent probes with AIE characteristic based on the change of covalent bond

基于共价键变化的靶标响应性AIE荧光探针

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Abstract

It is important for the determination of biologically and/or environmentally relevant species by utilization of fluorescent probes. Conventional fluorescent probes are subjected to the influence of aggregation-caused quenching that is limiting their application due to low selectivity as well as photobleaching. Additionally, quencher pairs are usually introduced in the design of these probes, which lead to the complex synthetic procedure. A novel class of fluorogens with aggregation-induced emission (AIE) characteristic provide a solution to address the dilemma. By taking advantage of the unique characteristic of AIE fluorogens, specific turn-on probes have been developed via combination with recognition components, exhibiting low background, good selectivity and outstanding photostability. This review focuses on the development of fluorescent probes with AIE characteristics via the bond cleavage as well as formation strategy.

摘要

荧光探针是检测生物或者环境中相关物质的重要手段. 传统 的荧光探针由于聚集诱导猝灭引起的选择性差异和容易光漂白等 问题限制了其应用. 此外, 在传统荧光探针的设计中通常引入猝灭 基团, 从而增加了探针合成及分离的难度. 一类新型的具有聚集诱 导发光(AIE)特征的荧光染料解决了上述问题. 利用AIE发光团的 独特性质, 构建发光团与识别基团偶联的特异性“关开型”探针, 这些探针具有背景低、选择性好和光稳定性强的优点. 本综述主要概括并讨论了通过共价键断裂以及新键形成策略开发的具有AIE特征的荧光探针, 并对其进行了展望.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21525523, 21722507, 21574048 and 21874121), the National Basic Research Program of China (973 Program, 2015CB932600), the National Key R&D Program of China (2017YFA0208000 and 2016YFF0100800), and the Natural Science Foundation of Zhejiang Province of China (LY18B050002).

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Authors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, China

    Min Xu  (许敏), Quan Wang  (王全), Qinyu Hu  (胡钦钰), Xiaoding Lou  (娄筱叮) & Fan Xia  (夏帆)

  2. Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China

    Xudong Wang  (汪旭东), Kaixun Huang  (黄开勋) & Fan Xia  (夏帆)

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  1. Min Xu  (许敏)
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  2. Xudong Wang  (汪旭东)
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  3. Quan Wang  (王全)
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  4. Qinyu Hu  (胡钦钰)
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  5. Kaixun Huang  (黄开勋)
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  6. Xiaoding Lou  (娄筱叮)
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  7. Fan Xia  (夏帆)
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Corresponding authors

Correspondence to Xiaoding Lou  (娄筱叮) or Fan Xia  (夏帆).

Additional information

Min Xu obtained her BSc degree from China University of Geosciences in 2017, and she is currently studying for her Master’s degree at China University of Geosciences under the supervision of Prof. Xiaoding Lou and Prof. Fan Xia. Her current research is focused on the fluorescent nanoprobes.

Xiaoding Lou received her BSc degree from Hubei Engineering University (China) in 2007. She studied organic chemistry at Wuhan University (China), where she received her PhD degree in 2012 under the supervision of Prof. Zhen Li. She then worked for one year as a research associate in Prof. Ben Zhong Tang’s group at Hong Kong University of Science and Technology. In 2013, she became an assistant professor and associate professor at Huazhong University of Science and Technology (China), respectively. In 2016, she worked at the University of California, Santa Barbara in Prof. Alan J. Heeger’s group. Since 2017, she has been a professor of analytical chemistry at China University of Geosciences (Wuhan). Her scientific interest is focused on the chemical and biosensor field.

Fan Xia received his BSc degree from Huazhong University of Science and Technology (HUST) in China in 2003. He studied physical chemistry at the Institute of Chemistry, Chinese Academy of Sciences (ICCAS), where he received his PhD degree in 2008 under the supervision of Prof. Lei Jiang. He then worked as a postdoctoral fellow in Prof. Alan J. Heeger’s group at the University of California, Santa Barbara. He joined HUST as part of the 1000 Young Talents Program in 2012 and became professor at HUST. He is currently a professor and Dean of Faculty of Materials Science and Chemistry, China University of Geosciences (Wuhan). His scientific interest is focused on bioanalytical chemistry.

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Xu, M., Wang, X., Wang, Q. et al. Analyte-responsive fluorescent probes with AIE characteristic based on the change of covalent bond. Sci. China Mater. 62, 1236–1250 (2019). https://doi.org/10.1007/s40843-019-9407-9

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