Abstract
Plant peroxidases participate in versatile biological processes and stress responses. Peroxidase activity significantly increased under drought stress in rubber tree. To identify the functions of peroxidase genes in response to drought stress, the full-length cDNA of HbPRX42 was isolated from rubber tree. The HbPRX42 contains 338 amino acid residues and a plant peroxidase-like superfamily domain. Phylogenetic analysis with Arabidopsis Class III peroxidases revealed that HbPRX42 shared high identities with AtPRX42. Although HbPRX42 was expressed in all tissues, it was preferentially expressed in flower and latex in rubber tree. HbPRX42 expression was significantly upregulated in leaves by drought stress. Moreover, light, mechanical wounding, H2O2, abscisic acid, ethylene, methyl jasmonic acid, and salicylic acid treatments also led to marked accumulation of HbPRX42 transcripts in leaves, too. However, HbPRX42 transcripts were downregulated by powdery mildew infection. In a word, these results indicated the involvement of HbPRX42 in both biotic and abiotic stress responses via multiple signaling pathways in rubber tree.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31270643, 31570591), the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-34-GW5), the Fundamental Research Funds for Rubber Research Institute, CATAS (1630022015013), and the Natural Science Foundation of Hainan province (20153135).
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Li-Feng Wang and Ji-Kun Wang have contributed equally to this work.
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Wang, LF., Wang, JK., An, F. et al. Molecular cloning and characterization of a stress responsive peroxidase gene HbPRX42 from rubber tree. Braz. J. Bot 39, 475–483 (2016). https://doi.org/10.1007/s40415-016-0263-1
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DOI: https://doi.org/10.1007/s40415-016-0263-1