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Bacterial community structures and biodegradation kinetic of Tiamulin antibiotic degrading enriched consortia from swine wastewater

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Abstract

The antibiotic tiamulin (TIA) is common and widely used medication for dysentery eradication in swine productions. Tiamulin persists in livestock manure, and its residues have been found in various environment. This work obtained four tiamulin-degrading enriched bacterial consortia from a covered anaerobic lagoon system and a stabilized pond system of swine farms. Tiamulin was efficiently removed by the enriched cultures at the concentrations between 2.5 and 200 mg/L, with a removal of 60.1–99.9% during 16 h and a degradation half-life of 4.5–15.7 h. The stabilized pond system cultured with taimulin solely could eliminate tiamulin at the highest rates. The logistic substrate degradation model fit most of the experimental data. Next-generation amplicon sequencing was conducted, and it was found that the bacterial community was significantly impacted by the inoculum source, nutrient addition, and high tiamulin concentrations. Principal coordinate analysis (PCoA) indicated the similarity of bacterial communities in the original enriched samples and the 2.5 mg/L tiamulin-removed cultures. The 200 mg/L consortia were rather different and became similar to the other 200 mg/L consortia from different sources and cultures without nutrient supplementation. Shannon and Simpson indices suggested a reduction in bacterial diversity at high concentrations. The microbes that had high growth in the most efficient enriched culture, or which were abundant in all samples, or which increased with higher tiamulin concentrations were likely to be the major tiamulin-degrading bacteria. This is the first report suggested the possible roles of Achromobacter, Delftia, Flavobacterium, Pseudomonas, and Stenotrophomonas in tiamulin degradation.

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Funding

This study was partially funded by Office of Higher Education Commission (OHEC) and the S&T Postgraduate Education, Research Development Office (PERDO) for the support of their research program in Hazardous Substance Management in the Agricultural Industry through the Center of Excellence on Hazardous Substance Management. One researcher was partially financial support from Chulalongkorn Graduate School Thesis Grant. Some instrument used in this study was acquired through the King Mongkut’s University of Technology Thonburi 55th Anniversary Commemorative Fund.

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Authors and Affiliations

  1. International Program in Hazardous Substance and Environmental Management, Graduate School, Chulalongkorn University, Bangkok, 10330, Thailand

    Xuan Thi Kim Nguyen

  2. Department of Natural Science, Faculty of Education, Bac Lieu University, Bac Lieu, 260000, Vietnam

    Xuan Thi Kim Nguyen

  3. Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Onruthai Pinyakong

  4. Center of Excellences on Hazardous Substance Management (HSM), Chulalongkorn University, Bangkok, 10330, Thailand

    Onruthai Pinyakong & Parinda Thayanukul

  5. Faculty of Engineering, Department of Environmental Engineering, King Mongkut’s University of Technology, Thonburi, 10140, Thailand

    Parinda Thayanukul

  6. Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Parinda Thayanukul

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  1. Xuan Thi Kim Nguyen
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  2. Onruthai Pinyakong
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  3. Parinda Thayanukul
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Correspondence to Parinda Thayanukul.

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Nguyen, X.T.K., Pinyakong, O. & Thayanukul, P. Bacterial community structures and biodegradation kinetic of Tiamulin antibiotic degrading enriched consortia from swine wastewater. J Environ Health Sci Engineer 17, 1121–1130 (2019). https://doi.org/10.1007/s40201-019-00426-2

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