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Lack of association between CYP27B1 gene polymorphism and type 2 diabetes mellitus in Iranian patients

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Abstract

Purpose

Type 2 diabetes mellitus (T2DM) is a global health problem with multiple etiological factors. Previous studies indicated that 1- alpha, 25-dihydroxyvitamin D3, a molecule that is produced by CYP27B1, could protect insulin-secreted cells from destruction by immune cells. The aim of the study was to investigate the CYP27B1 promoter gene polymorphism in T2DM.

Methods

Two hundred subjects including 100 T2DM and 100 healthy individuals were recruited in the study. ARMS-PCR technique was used to identify rs10877012 genotypes in the 5′ region of CYP27B1.

Results

The frequency of CC, CA, and AA genotype was 61/50, 31/39, and 8/11, respectively in T2DM patients compared to healthy subjects. There was no significant difference between both groups in regarding to genotype and allele distribution (P > 0.05). CA (RR = 1.535, 95% CI = 0.841- 2.802) and AA (1.677, 95% CI = 0.627-4.490) genotypes had no association with increased risk of T2DM. In addition, CA + AA versus CC showed no increased risk for T2DM (RR = 0.639, 95% CI = 0.365-1.121).

Conclusion

We found no association between rs10877012 polymorphism and T2DM. There was no increased risk of this polymorphism in T2DM. Further studies with large groups are suggested in other populations to better understand the relation of CYP27B1 gene variation, especially its ethnicity-dependent relation with T2DM.

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Data would be available upon a reasonable request.

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Acknowledgments

This study was supported by vice chancellery of research affairs of Jahrom University of Medical Sciences.

Funding

This study was supported by Jahrom University of Medical Sciences.

Author information

Authors and Affiliations

  1. Department of Tissue Engineering, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran

    Saiedeh Erfanian

  2. Department of Biochemistry, Department of Advanced Medical Sciences and Technologies, Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Ostad motahhari Blvd, POBox 74148-46199, Jahrom, Iran

    Abazar Roustazadeh

Authors
  1. Saiedeh Erfanian
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  2. Abazar Roustazadeh
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Contributions

AR supervised the study and wrote the manuscript.SE assisted in laboratory measurements and manuscript writing.

Corresponding author

Correspondence to Abazar Roustazadeh.

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Ethics committee of Jahrom University of Medical sciences approved the study.

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None for all authors.

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Erfanian, S., Roustazadeh, A. Lack of association between CYP27B1 gene polymorphism and type 2 diabetes mellitus in Iranian patients. J Diabetes Metab Disord 21, 517–520 (2022). https://doi.org/10.1007/s40200-022-01003-5

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  • DOI: https://doi.org/10.1007/s40200-022-01003-5

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