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Cadmium and Lead Bioaccumulation During Growth Stages Alters Sugar and Vitamin C Content in Dietary Vegetables

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Abstract

A field experiment was designed to investigate the effects of cadmium and lead on biomass production, sugar and vitamin C content of dietary vegetables. At seedling stage the reduction magnitudes in biomass production with added cadmium up to 50 mg kg−1 soils were 49–51 % in fenugreek root and 32.2–39.6 % in spinach leaf; while at 40 days after sowing (vegetative growth stage); they were observed as 36.1–47.2 % in spinach root and 17.8–20 % in coriander leaf. Cadmium caused maximum reduction in sugar content (62 %) in radish leaf and vitamin C content (56.4 %) in coriander leaf at seedling stage; whereas, maximum reduction in sugar content (66.7 %) and vitamin C content (59.4 %) was observed in spinach leaf and coriander leaf, respectively, under the combined treatment (Cd 50 mg kg−1 + Pb 500 mg kg−1) at vegetative growth stage. The dietary vegetables enhance their antioxidant activity against metal stress when applied below the critical level, however, dosage of Cd higher than critical level (≥25 mg kg−1 soil in treatments II, III and IX) drastically alters plant growth (stunted), reduced yield as well as dietary contents (sugar and vitamin C) of these important vegetables especially it’s antioxidant content; and the hazardous effect was more visible at higher bioaccumulation of heavy metals during vegetative growth stage. It is concluded that dietary vegetables should be utilized for human consumption before the vegetative growth stage especially in the soils polluted with cadmium and lead in order to minimize the intake of pollutants.

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References

  1. Ifron ET, Basir O (1979) Nutritive value of some Nigerian leafy vegetables part 3. Food Chem 5:231–235

    Article  Google Scholar 

  2. Gockowski J, Mbazo’o G, Mbah TF (2003) African traditional leafy vegetables and the urban and peri-urban poor. Food Policy 28:221–235

    Article  Google Scholar 

  3. Szeto YT, Tomlinson B, Benzie IF (2002) Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation. Br J Nutr 87(1):55–59

    Article  PubMed  CAS  Google Scholar 

  4. Igbal K, Khan A, Khattak MAK (2004) Biological significance of ascorbic acid (vitamin C) in human health-a review. Pakistan J Nutri 3(1):5–13

    Article  Google Scholar 

  5. Gholamabbas S, Majid A, Sayed FM, Karim CA, Brian KR, Rainer S (2010) Transport of Cd, Cu, Pb and Zn in a calcareous soil under wheat and safflower cultivation––a column study. Geoderma 154:311–320

    Article  Google Scholar 

  6. Angelova A, Ivanova K, Ivanova R (2004) Effect of chemical forms of lead, cadmium, and zinc in polluted soils on their uptake by tobacco. J Plant Nutri 27(5):757–773

    Article  CAS  Google Scholar 

  7. Yusuf AA, Arowolo TA, Bamgbose O (2003) Cadmium, copper and nickel levels in vegetables from industrial and residential areas of Lagos City, Nigeria. Food Chem Toxicol 41:375–378

    Article  PubMed  CAS  Google Scholar 

  8. Gupta UC, Gupta SC (1998) Trace element toxicity relationships to crop production and livestock and human health: implications for management. Commun Soil Sci Plant Anal 29:1491–1522

    Article  CAS  Google Scholar 

  9. Bolan NS, Duraisamy VP (2003) Role of inorganic and organic soil amendments on immobilization and phyto-availability of heavy metals: a review involving specific case studies. Aust J Soil Res 41:533–555

    Article  CAS  Google Scholar 

  10. Carlos A, Constantin L, Garcia FP, Razo LMD, Vazquez RR, Varaldo HMP (2005) Chemical fraction of heavy metals in soils irrigated with waste water in central Mexico. Agric Eco Environ 108:57–71

    Article  Google Scholar 

  11. Vassilev A, Perez SA, Semane B, Carleer R, Vangronsveld J (2005) Cadmium accumulation and tolerance of two salix genotypes hydrophonically grown in presence of cadmium. J Plant Nutri 28:2159–2177

    Article  CAS  Google Scholar 

  12. Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci Soc Am J 42:421–428

    Article  CAS  Google Scholar 

  13. Walkley A, Black AI (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modification of chromic acid titration method. Soil Sci 37:29–38

    Article  CAS  Google Scholar 

  14. Chopra SL, Kanwar JS (1999) Analytical agricultural chemistry. Kalyani Publication, New Delhi

    Google Scholar 

  15. Kumar C, Mani D (2010) Enrichment and management of heavy metals in sewage-irrigated soil. Lap LAMBERT Acad Publishing, Dudweiler (Germany)

    Google Scholar 

  16. Allen SE, Grimshaw HM, Rowland AP (1986) Chemical analysis. In: Moore PD, Chapman SB (eds) Methods in plant ecology. Blackwell Scientific Publication, London, pp 285–344

    Google Scholar 

  17. Al-Ani M, Opara LU, Al-Bahri D, Al-Rahbi N (2007) Spectrophotometric quantification of ascorbic acid contents of fruit and vegetables using the 2,4-dinitrophenylhydrazine method. J Food Agric Environ 5(3 &4):165–168

    CAS  Google Scholar 

  18. Kharn MMR, Rahman MM, Islam MS, Begum SA (2006) A simple UV-spectrophotometric method for the determination of vitamin c content in various fruits and vegetables at Sylhate area in Bangladesh. J Biol Sci 6(2):388–392

    Article  Google Scholar 

  19. Qingling Fu, Hongqing Hu, Li Jiajia, Li Huang, Haizheng Y, Lv Yi (2009) Effects of soil polluted by cadmium and lead on production and quality of pepper (Capsicum annuum L.) and radish (Raphanus sativus L.). J Food Agric Environ 7(2):698–702

    Google Scholar 

  20. Li X, Coles BJ, Ramsey MH, Thornton I (1995) Sequential extraction of soils for multi-element analysis by ICP-AES. Chem Geol 124:109–123

    Article  CAS  Google Scholar 

  21. Dang YP, Chhabra R, Verma KS (1990) Effect of Cd, Ni, Pb and Zn on growth and composition of onion and fenugreek. Commun Soil Sci Plant Anal 21(9/10):717–735

    Article  CAS  Google Scholar 

  22. Pandey N, Sharma CP (2002) Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Sci 163:753–758

    Article  CAS  Google Scholar 

  23. Muhammad A, Zahida P, Muhammad I, Riazuddin Iqbal S, Ahmed M, Bhutto R (2010) Monitoring of toxic metals (cadmium, lead, arsenic and mercury) in vegetables of Sindh Pakistan. Kathmandu Univ J Sci Eng Technol 6(2):60–65

    Google Scholar 

  24. Padmaja K, Prasad DDK, Prasad ARK (1990) Inhibition of chlorophyll synthesis in Phaseolus vulgaris seedling by cadmium acetate. Photosynthetica 24:399–405

    CAS  Google Scholar 

  25. Arduini I, Godbold DL, Onnis A (1996) Cadmium and copper uptake and distribution in Mediterranean tree seedlings. Physiol Plant 97:111–117

    Article  CAS  Google Scholar 

  26. Haluskov L, Valentovicova K, Huttova J, Mistrik I, Tamas L (2010) Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen production in barley root tip. Plant Growth Regul 62(1):59–64

    Article  Google Scholar 

  27. John R, Ahmad P, Gadgil K, Sharma S (2009) Heavy metal toxicity: effect on plant growth biochemical parameters and metal accumulation by Brassica juncea L. Int J Plant Prod 3(3):65–75

    CAS  Google Scholar 

  28. Fang-YI Chu, Sun JIE, Xianzhong WU, Rui Hai LIU (2002) Antioxidant and anti-proliferative activities of common vegetables. J Agric Food Chem 50:6910–6916

    Article  Google Scholar 

  29. Rajeshwari CU, Andallu B (2011) Oxidative stress in NIDDM patients: influence of coriander (Coriandrum sativum) seeds. Res J Pharm Biol Chem Sci 2(1):31

    Google Scholar 

  30. Ogunlesi M, Okiei W, Azeez L, Obakachi V, Osunsanmi M, Nkenchor G (2010) Vitamin C contents of tropical vegetables and foods determined by voltametric and titrimetric methods and their relevance to the medicinal uses of the plants. Int J Electrochem Sci 5:105–115

    CAS  Google Scholar 

  31. Girisha ST, Ragavendra BV (2009) Accumulation of heavy metals in leafy vegetables grown in urban areas by using sewage water and its effect. Arch Phytopathol Plant Prot 42(10):956–959

    Article  CAS  Google Scholar 

  32. Abbas M, Parveen Z, Iqbal M, Riazuddin Iqbal S, Ahmed M, Bhutto R (2010) Monitoring of toxic metals (cadmium, lead, arsenic and mercury) in vegetables of spinach, Pakistan. Kathmandu Univ J Sci Eng Technol 6(2):60–65

    Google Scholar 

  33. Jiang W, Liu D, Hou W (2000) Hyperaccumulation of lead by roots hypocotyles and shoots of Brassica juncea. Biol Plant 43(4):603–606

    Article  CAS  Google Scholar 

  34. Bigdeli M, Seilsepour M (2008) Investigation of metals accumulation in some vegetables irrigated with waste water in Shahre Rey-Iron and toxicological implications. American-Eurasian J Agric Environ 4(1):86–92

    Google Scholar 

  35. Singh RP, Agrawal M (2007) Effect of sewage sludge amendment on heavy metal accumulation and consequent responses of Beta vulgaris plants. Chemosphere 67:2229–2240

    Article  PubMed  CAS  Google Scholar 

  36. Sharma RK, Agrawal M, Marshall F (2006) Heavy metals contamination in vegetables grown in waste water irrigated areas of Varanai, India. Bull Environ Contam Toxicol 77:312–318

    Article  PubMed  CAS  Google Scholar 

  37. Ismail BS, Farihah K, Khairiah J (2005) Bioaccumulation of heavy metals in vegetables from selected agricultural areas. Bull Environ Contam Toxicol 74:320–327

    Article  PubMed  CAS  Google Scholar 

  38. Hounsome N, Hounsome B, Tomos D, Edwards JG (2008) Plant metabolites and nutritional quality of vegetables. J Food Sci 73(4):48–65

    Article  Google Scholar 

  39. Singh RK, Sudhakar A, Lokeshwar BL (2011) From normal cells to malignancy: distinct role of pro-inflammatory factors and cellular redox mechanisms. J Cancer Sci Ther 03:70–75

    CAS  Google Scholar 

  40. Al-Madani WA, Siddiqi NJ, Alhomida AS (2009) Renal toxicity of mercuric chloride at different time intervals in rats. Biochem Insights 02:37–45

    CAS  Google Scholar 

  41. Mani D, Sharma B, Kumar C (2007) Phytoaccumulation, interaction, toxicity and remediation of cadmium from Helianthus annuus L. (sunflower). Bull Environ Contam Toxicol 79:71–79

    Article  PubMed  CAS  Google Scholar 

  42. UNCTAD (2003) Organic fruit and vegetables from the Tropic, United Nations Conference on Trade & Development (UNCTAD), Geneva 10, Switzerland, 203–210

  43. Mani D, Kumar C, Srivastava RK (2007) Effect of calcium, zinc and organic matter on the uptake of cadmium in Brassica rapa L. (turnip). Proc Natl Acad Sci India 77(2):263–276

    Google Scholar 

Download references

Acknowledgments

The research was supported by The National Academy of Sciences, India (Allahabad), the Sheila Dhar Memorial Scholarship Fund and the Indian Farmer Fertilizer Cooperative (IFFCO) Ltd., Phulpur Unit, Ghiya Nagar, Phulpur Allahabad-212404, Uttar Pradesh, India. Opinions in the paper do not constitute an approval by the funding agencies but only reflect the personal views of the authors.

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Authors and Affiliations

  1. Sheila Dhar Institute of Soil Science, Department of Chemistry, University of Allahabad, 5 Lajpatrai Road, Allahabad, 211002, UP, India

    Dinesh Mani & Shiv Balak

  2. Department of Biochemistry, University of Allahabad, Allahabad, 211002, India

    Bechan Sharma

  3. DNA Club Project of DBT, The National Academy of Science, India, Allahabad, 211002, India

    Chitranjan Kumar

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  1. Dinesh Mani
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  2. Bechan Sharma
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Correspondence to Chitranjan Kumar.

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Mani, D., Sharma, B., Kumar, C. et al. Cadmium and Lead Bioaccumulation During Growth Stages Alters Sugar and Vitamin C Content in Dietary Vegetables. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 82, 477–488 (2012). https://doi.org/10.1007/s40011-012-0057-6

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