Abstract
A biosurfactant producing isolate of Stenotrophomonas maltophilia (BR-12) was isolated from crude oil-contaminated soil from Vadodara oil refinery (India) and characterized on the basis of biochemical tests and identified by 16S rRNA sequencing. It was also assessed for its Pyrene bioremediation potential under submerged conditions in minimal media spiked with Pyrene at 20, 50, and 100 µg mL−1. Analysis of microbial growth parameters revealed that BR-12 grew best at 50 µg mL−1 Pyrene concentration, leading to 87 % degradation of Pyrene within 20 days and production of high amount of biosurfactant (2.2 g L−1). Since biosurfactant production ability of bacteria can play a major role in enhancing its ability to degrade Pyrene, this promising strain needs to be further exploited in bioremediation of PAH-contaminated sites.
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The financial assistance provided by Indian council of Agricultural Research, New Delhi through AMAAS network project and facilities provided by Division of Microbiology, IARI, New Delhi, are gratefully acknowledged.
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Singh, A., Kumar, K., Pandey, A.K. et al. Pyrene Degradation by Biosurfactant Producing Bacterium Stenotrophomonas maltophilia . Agric Res 4, 42–47 (2015). https://doi.org/10.1007/s40003-014-0144-4
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DOI: https://doi.org/10.1007/s40003-014-0144-4