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Wicking Property of Graft Material Enhanced Bone Regeneration in the Ovariectomized Rat Model

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Recruitment and homing cells into graft materials from host tissue is crucial for bone regeneration.

Methods:

Highly porous, multi-level structural, hydroxyapatite bone void filler (HA-BVF) have been investigated to restore critical size bone defects. The aim was to investigate a feasibility of bone regeneration of synthetic HA-BVF compared to commercial xenograft (Bio-Oss). HA-BVF of 0.7 mm in average diameter was prepared via template coating method. Groups of animals (n = 6) were divided into two with normal (Sham) or induced osteoporotic conditions (Ovx). Subsequently, subdivided into three treated with HA-BVF as an experiment or Bio-Oss as a positive control or no treatment as a negative control (defect). The new bone formation was analyzed by micro-CT and histology.

Results:

At 4 weeks post-surgery, new bone formation was initiated from all groups. At 8 weeks post-surgery, new bone formation in the HA-BVF groups was greater than Bio-Oss groups. Extraordinarily greater bone regeneration within the Ovx-HA group than Sham–Bio-Oss or Ovx–Bio-Oss group (p < 0.05).

Conclusion:

This study suggests that the immediate wicking property of HA-BVF from host tissue activates a natural healing cascade without the addition of exogeneous factors or progenitor cells. HA-BVF may be an effective alternative for repairing bone defects under both normal and osteoporotic bone conditions.

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Acknowledgements

This study was partially supported by Chonnam National University, 2016 and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Education (2017R1D1A1B03034829).

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Authors and Affiliations

  1. College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Gwangju, 61186, Republic of Korea

    Seunghyun Kim, Taeho Ahn & Chunsik Bae

  2. Department of Chemical Engineering, Kyungil University, 50 Gamasil-gil, Gyeongsan, 38428, Republic of Korea

    Myung-Ho Han

  3. Carroll Laboratory for Orthopedic Surgery, Columbia University, 650 West 168th Street, New York, NY, 10032, USA

    Daniel S. Oh

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  1. Seunghyun Kim
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  2. Taeho Ahn
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Correspondence to Chunsik Bae or Daniel S. Oh.

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Ethical statement

Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Chonnam National University (CNU IACUC-YB-R-2014-37) and the animals were cared for in accordance with the Guidelines for Animal Experiments of Chonnam National University.

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Kim, S., Ahn, T., Han, MH. et al. Wicking Property of Graft Material Enhanced Bone Regeneration in the Ovariectomized Rat Model. Tissue Eng Regen Med 15, 503–510 (2018). https://doi.org/10.1007/s13770-018-0142-x

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  • DOI: https://doi.org/10.1007/s13770-018-0142-x

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